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dc.contributor.authorBoni-Mitake, M.-
dc.contributor.authorCosta, H.-
dc.contributor.authorSpencer, P. J.-
dc.contributor.authorVassilieff, V. S.-
dc.contributor.authorRogero, J. R.-
dc.date.accessioned2014-05-20T15:11:32Z-
dc.date.accessioned2016-10-25T17:45:50Z-
dc.date.available2014-05-20T15:11:32Z-
dc.date.available2016-10-25T17:45:50Z-
dc.date.issued2001-12-01-
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2001001200004-
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 12, p. 1531-1538, 2001.-
dc.identifier.issn0100-879X-
dc.identifier.urihttp://hdl.handle.net/11449/28066-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/28066-
dc.description.abstractIonizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide (pI 10.3) from Crotalus durissus terrificus venom composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of the present study was to carry out a biochemical study and a toxic activity assay on native and irradiated crotamine. Crotamine was purified from C.d. terrificus venom by Sephadex G-100 gel filtration followed by ion-exchange chromatography, and irradiated at 2 mg/ml in 0.15 M NaCl with 2.0 kGy gamma radiation emitted by a 60Co source. The native and irradiated toxins were evaluated in terms of structure and toxic activity (LD50). Irradiation did not change the protein concentration, the electrophoretic profile or the primary structure of the protein although differences were shown by spectroscopic techniques. Gamma radiation reduced crotamine toxicity by 48.3%, but did not eliminate it.en
dc.format.extent1531-1538-
dc.language.isoeng-
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)-
dc.sourceSciELO-
dc.subjectcrotamineen
dc.subjectgamma radiationen
dc.subjectchromatographyen
dc.subjecttoxicityen
dc.subjectcircular dichroismen
dc.titleEffects of 60Co gamma radiation on crotamineen
dc.typeoutro-
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares (IPEN)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationInstituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN/SP) Departamento de Radioproteção Ocupacional-
dc.description.affiliationInstituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN/SP) Departamento de Bioengenharia-
dc.description.affiliationInstituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN/SP) Diretoria de Materiais-
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Ceatox - Centro de Assistência Toxicológica-
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Ceatox - Centro de Assistência Toxicológica-
dc.identifier.doi10.1590/S0100-879X2001001200004-
dc.identifier.scieloS0100-879X2001001200004-
dc.identifier.wosWOS:000172765500004-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileS0100-879X2001001200004.pdf-
dc.relation.ispartofBrazilian Journal of Medical and Biological Research-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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