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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/29025
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dc.contributor.authorPereira-Junior, Oduvaldo Câmara Marques-
dc.contributor.authorRahal, Sheila Canevese-
dc.contributor.authorLima-Neto, João Ferreira-
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz-
dc.contributor.authorMonteiro, Frederico Ozanan Barros-
dc.date.accessioned2014-05-20T15:14:03Z-
dc.date.accessioned2016-10-25T17:47:59Z-
dc.date.available2014-05-20T15:14:03Z-
dc.date.available2016-10-25T17:47:59Z-
dc.date.issued2013-05-01-
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000500006-
dc.identifier.citationActa Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.-
dc.identifier.issn0102-8650-
dc.identifier.urihttp://hdl.handle.net/11449/29025-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/29025-
dc.description.abstractPURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.format.extent353-360-
dc.language.isoeng-
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia-
dc.sourceSciELO-
dc.subjectHydroxyapatitesen
dc.subjectBone Matrixen
dc.subjectCastor Oilen
dc.subjectPolyurethanesen
dc.subjectStem Cellsen
dc.titleIn vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cellsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionFederal Rural University of the Amazon Institute of Animal Health and Production-
dc.description.affiliationUNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology-
dc.description.affiliationUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology-
dc.description.affiliationFederal Rural University of the Amazon Institute of Animal Health and Production-
dc.description.affiliationUnespUNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology-
dc.description.affiliationUnespUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology-
dc.identifier.doi10.1590/S0102-86502013000500006-
dc.identifier.scieloS0102-86502013000500006-
dc.identifier.wosWOS:000319994200006-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileS0102-86502013000500006.pdf-
dc.relation.ispartofActa Cirúrgica Brasileira-
dc.identifier.orcid0000-0002-9211-4093pt
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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