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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/2927
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dc.contributor.authorOliveira, GH-
dc.contributor.authorBerchieri Jr, A-
dc.contributor.authorMontassier, Helio Jose-
dc.date.accessioned2014-05-20T13:15:55Z-
dc.date.available2014-05-20T13:15:55Z-
dc.date.issued2006-03-01-
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2006000100008-
dc.identifier.citationRevista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.-
dc.identifier.issn1516-635X-
dc.identifier.urihttp://hdl.handle.net/11449/2927-
dc.description.abstractThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.en
dc.format.extent51-54-
dc.language.isoeng-
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas-
dc.sourceSciELO-
dc.subjectChickenen
dc.subjectELISAen
dc.subjectenzymeen
dc.subjectSalmonella spen
dc.subjectSerologyen
dc.titleChicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisaen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNESP FCAV Department of Animal Pathology-
dc.description.affiliationUnespUNESP FCAV Department of Animal Pathology-
dc.identifier.doi10.1590/S1516-635X2006000100008-
dc.identifier.scieloS1516-635X2006000100008-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileS1516-635X2006000100008.pdf-
dc.relation.ispartofRevista Brasileira de Ciência Avícola-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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