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dc.contributor.authorOliveira Amorim, Jose Benedito-
dc.contributor.authorMusa-Aziz, Raif-
dc.contributor.authorLessa, Lucilia M. A.-
dc.contributor.authorMalnic, Gerhard-
dc.contributor.authorFonteles, Manasses Claudino-
dc.date.accessioned2014-05-20T15:21:05Z-
dc.date.accessioned2016-10-25T17:54:22Z-
dc.date.available2014-05-20T15:21:05Z-
dc.date.available2016-10-25T17:54:22Z-
dc.date.issued2006-10-01-
dc.identifierhttp://dx.doi.org/10.1139/Y06-044-
dc.identifier.citationCanadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 84, n. 10, p. 1003-1010, 2006.-
dc.identifier.issn0008-4212-
dc.identifier.urihttp://hdl.handle.net/11449/32267-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/32267-
dc.description.abstractThe effect of uroguanylin (UGN) oil K(+) and H(+) secretion in the renal tubules of the rat kidney was studied using in vivo stationary microperfusion. For the study of K(+) secretion, a tubule was Punctured to inject a column of FDC-green-colored Ringer's solution with 0.5 mmol KCI/L 10(-6)(mol UGN/L, and oil was Used to block fluid flow. K(+) activity and transepithelial potential differences (PD) were measured with double microelectrodes (K(+) ion-selective resin vs. reference) in the distal tubules of the same nephron. During perfusion, K(+) activity rose exponentially, from 0.5 mmol/L to stationary concentration, allowing for the calculation of K(+) secretion J(K)). JK increased from 0.63 +/- 0.06 nmol.cm(-2).s(-1) in the control croup to 0.85 +/- 0.06 in the UGN group (p < 0.01). PD was -51.0 +/- 5.3 mV in the control group and -50.3 +/- 4.98 mV in the UGN group. In the presence of 10(-7) mol iberiotoxin/L, the UGN effect was abolished: JK was 0.37 +/- 0.038 nmol-cm(-2).s(-1) in the absence of, and 0.38 +/- 0.025 in the presence of, UGN. indicating its action oil rnaxi-K channels. In another series of experiments, renal tubule acidification was studied, using similar method: proximal and distal tubules were perfused with solutions containing 25 mmol NaHCO(3)/L. Acidification half-time was increased both in proximal and distal segments and, as a consequence, bicarbonate reabsorption decreased in the presence of UGN (in proximal tubules, from 2.40 +/- 0.26 to 1.56 +/- 0.21 nmol-cm(-2).s(-1)). When the Na(+)/H(+) exchanger was inhibited by 10(-4) mol hexamethylene amiloride (HMA)/L, the control and UGN groups were not significantly different. In the late distal tubule, after HMA, UGN significantly reduced J(HCO3)(-). indicating all effect of UGN oil H(+)-ATPase. These data show that UGN stimulated J(K)(+) by actin, oil maxi-K channels. and decreased J(HCO3)(-) by acting on NHE3 in proximal and H(+)-ATPase in distal tubules.en
dc.format.extent1003-1010-
dc.language.isoeng-
dc.publisherNatl Research Council Canada-n R C Research Press-
dc.sourceWeb of Science-
dc.subjectrenal tubulespt
dc.subjectmicroperfusionpt
dc.subjectpHpt
dc.subjectmaxi K channelspt
dc.subjectNa(+)/H(+)pt
dc.subjectH-ATPasept
dc.titleEffect of uroguanylin on potassium and bicarbonate transport in rat renal tubulesen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniv Presbiteriana Mackenzie & Estadual Ceara-
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Dept Fisiol & Biofis, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUNESP, Fac Odontol, Sao Jose Dos Campos, Brazil-
dc.description.affiliationUniv Presbiteriana Mackenzie & Estadual Ceara, BR-01302907 São Paulo, Brazil-
dc.description.affiliationUnespUNESP, Fac Odontol, Sao Jose Dos Campos, Brazil-
dc.identifier.doi10.1139/Y06-044-
dc.identifier.wosWOS:000243731100006-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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