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DC Field | Value | Language |
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dc.contributor.author | Oliveira Amorim, Jose Benedito | - |
dc.contributor.author | Musa-Aziz, Raif | - |
dc.contributor.author | Lessa, Lucilia M. A. | - |
dc.contributor.author | Malnic, Gerhard | - |
dc.contributor.author | Fonteles, Manasses Claudino | - |
dc.date.accessioned | 2014-05-20T15:21:05Z | - |
dc.date.accessioned | 2016-10-25T17:54:22Z | - |
dc.date.available | 2014-05-20T15:21:05Z | - |
dc.date.available | 2016-10-25T17:54:22Z | - |
dc.date.issued | 2006-10-01 | - |
dc.identifier | http://dx.doi.org/10.1139/Y06-044 | - |
dc.identifier.citation | Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 84, n. 10, p. 1003-1010, 2006. | - |
dc.identifier.issn | 0008-4212 | - |
dc.identifier.uri | http://hdl.handle.net/11449/32267 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/32267 | - |
dc.description.abstract | The effect of uroguanylin (UGN) oil K(+) and H(+) secretion in the renal tubules of the rat kidney was studied using in vivo stationary microperfusion. For the study of K(+) secretion, a tubule was Punctured to inject a column of FDC-green-colored Ringer's solution with 0.5 mmol KCI/L 10(-6)(mol UGN/L, and oil was Used to block fluid flow. K(+) activity and transepithelial potential differences (PD) were measured with double microelectrodes (K(+) ion-selective resin vs. reference) in the distal tubules of the same nephron. During perfusion, K(+) activity rose exponentially, from 0.5 mmol/L to stationary concentration, allowing for the calculation of K(+) secretion J(K)). JK increased from 0.63 +/- 0.06 nmol.cm(-2).s(-1) in the control croup to 0.85 +/- 0.06 in the UGN group (p < 0.01). PD was -51.0 +/- 5.3 mV in the control group and -50.3 +/- 4.98 mV in the UGN group. In the presence of 10(-7) mol iberiotoxin/L, the UGN effect was abolished: JK was 0.37 +/- 0.038 nmol-cm(-2).s(-1) in the absence of, and 0.38 +/- 0.025 in the presence of, UGN. indicating its action oil rnaxi-K channels. In another series of experiments, renal tubule acidification was studied, using similar method: proximal and distal tubules were perfused with solutions containing 25 mmol NaHCO(3)/L. Acidification half-time was increased both in proximal and distal segments and, as a consequence, bicarbonate reabsorption decreased in the presence of UGN (in proximal tubules, from 2.40 +/- 0.26 to 1.56 +/- 0.21 nmol-cm(-2).s(-1)). When the Na(+)/H(+) exchanger was inhibited by 10(-4) mol hexamethylene amiloride (HMA)/L, the control and UGN groups were not significantly different. In the late distal tubule, after HMA, UGN significantly reduced J(HCO3)(-). indicating all effect of UGN oil H(+)-ATPase. These data show that UGN stimulated J(K)(+) by actin, oil maxi-K channels. and decreased J(HCO3)(-) by acting on NHE3 in proximal and H(+)-ATPase in distal tubules. | en |
dc.format.extent | 1003-1010 | - |
dc.language.iso | eng | - |
dc.publisher | Natl Research Council Canada-n R C Research Press | - |
dc.source | Web of Science | - |
dc.subject | renal tubules | pt |
dc.subject | microperfusion | pt |
dc.subject | pH | pt |
dc.subject | maxi K channels | pt |
dc.subject | Na(+)/H(+) | pt |
dc.subject | H-ATPase | pt |
dc.title | Effect of uroguanylin on potassium and bicarbonate transport in rat renal tubules | en |
dc.type | outro | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ Presbiteriana Mackenzie & Estadual Ceara | - |
dc.description.affiliation | Univ São Paulo, Inst Ciências Biomed, Dept Fisiol & Biofis, BR-05508900 São Paulo, Brazil | - |
dc.description.affiliation | UNESP, Fac Odontol, Sao Jose Dos Campos, Brazil | - |
dc.description.affiliation | Univ Presbiteriana Mackenzie & Estadual Ceara, BR-01302907 São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP, Fac Odontol, Sao Jose Dos Campos, Brazil | - |
dc.identifier.doi | 10.1139/Y06-044 | - |
dc.identifier.wos | WOS:000243731100006 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Canadian Journal of Physiology and Pharmacology | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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