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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/32319
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dc.contributor.authorKeid, L. B.-
dc.contributor.authorSoares, R. M.-
dc.contributor.authorVieira, N. R.-
dc.contributor.authorMegid, Jane-
dc.contributor.authorSalgado, V. R.-
dc.contributor.authorVasconcellos, S. A.-
dc.contributor.authorda Costa, M.-
dc.contributor.authorGregori, F.-
dc.contributor.authorRichtzenhain, L. J.-
dc.date.accessioned2014-05-20T15:21:08Z-
dc.date.accessioned2016-10-25T17:54:27Z-
dc.date.available2014-05-20T15:21:08Z-
dc.date.available2016-10-25T17:54:27Z-
dc.date.issued2007-11-01-
dc.identifierhttp://dx.doi.org/10.1007/s11259-006-0109-6-
dc.identifier.citationVeterinary Research Communications. Dordrecht: Springer, v. 31, n. 8, p. 951-965, 2007.-
dc.identifier.issn0165-7380-
dc.identifier.urihttp://hdl.handle.net/11449/32319-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/32319-
dc.description.abstractA pair of primers directed to 16S-23S rDNA interspacer (ITS) was designed directed to Brucella genetic sequences in order to develop a polymerase chain reaction (PCR) putatively capable of amplifying DNA from any Brucella species. Nucleic acid extracts from whole-blood from naive dogs were spiked with decreasing amounts of Brucella canis RM6/66 DNA and the resulting solutions were tested by PCR. In addition, the ability of PCR to amplify Brucella spp. genetic sequences from naturally infected dogs was evaluated using 210 whole-blood samples of dogs from 19 kennels. The whole-blood samples collected were subjected to blood culture and PCR. Serodiagnosis was performed using the rapid slide agglutination test with and without 2-mercaptoethanol. The DNA from whole blood was extracted using proteinase-K, sodium dodecyl sulphate and cetyl trimethyl ammonium bromide followed by phenol-chloroform purification. The PCR was capable of detecting as little as 3.8 fg of Brucella DNA mixed with 450 ng of host DNA. Theoretically, 3.8 fg of Brucella DNA represents the total genomic mass of fewer than two bacterial cells. The PCR diagnostic sensitivity and specificity were 100%. From the results observed in the present study, we conclude that PCR could be used as confirmatory test for diagnosis of B. canis infection.en
dc.format.extent951-965-
dc.language.isoeng-
dc.publisherSpringer-
dc.sourceWeb of Science-
dc.subjectBrucella canispt
dc.subjectcanine brucellosispt
dc.subjectPCRpt
dc.subjectinternal transcribed spacerpt
dc.subjectblood culturept
dc.subjectrapid slide agglutination testpt
dc.titleDiagnosis of canine brucellosis: Comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspaceren
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)-
dc.description.affiliationUniv São Paulo, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, BR-05508 São Paulo, Brazil-
dc.description.affiliationSão Paulo State Univ, Sch Vet Med, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil-
dc.description.affiliationUniv Fed Rio Grande do Sul, Hlth Sci Inst, Dept Microbiol, BR-90046900 Porto Alegre, RS, Brazil-
dc.description.affiliationInst Biol, Res & Dev Ctr Anim Hlth, São Paulo, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Sch Vet Med, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil-
dc.identifier.doi10.1007/s11259-006-0109-6-
dc.identifier.wosWOS:000247931900003-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Research Communications-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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