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DC Field | Value | Language |
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dc.contributor.author | VALENTINI, SR | - |
dc.contributor.author | Oliveira, MLS | - |
dc.contributor.author | Sasahara, R. M. | - |
dc.contributor.author | Armelin, MCS | - |
dc.date.accessioned | 2014-05-20T15:23:46Z | - |
dc.date.accessioned | 2016-10-25T17:57:45Z | - |
dc.date.available | 2014-05-20T15:23:46Z | - |
dc.date.available | 2016-10-25T17:57:45Z | - |
dc.date.issued | 1994-02-01 | - |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_issues&pid=0100-879X&lng=en&nrm=iso | - |
dc.identifier.citation | Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 27, n. 2, p. 541-546, 1994. | - |
dc.identifier.issn | 0100-879X | - |
dc.identifier.uri | http://hdl.handle.net/11449/34474 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/34474 | - |
dc.description.abstract | Glucocorticoid hormones modulate the actions of peptide growth factors and constitute important therapeutic tools as anti-inflammatory and anti-tumor agents. The C6 rat glioma cell line responds to glucocorticoids with changes in morphology and growth block. The hyper-responsive ST1 cell variant displays a dramatic phenotypic reversion under the influence of these hormones. Thus, the transformed and tumorigenic cells reversibly change to a normal and non-tumorigenic phenotype. In addition, the cells also produce a C-type retrovirus. We used poly A(+) mRNA from ST1 cells that had been treated with hydrocortisone to generate a cDNA library that was then screened, by differential hybridization,for glucocorticoid-responsive cellular sequences. The retroviral genomic RNA was used to generate a viral-specific probe. Cross hybridization led to the isolation of at least 4 cDNA clones of which 3 are cellular sequences and one corresponds to a retroviral gene. These clones were characterized by DNA sequencing and Northern blot hybridization analysis. | en |
dc.format.extent | 541-546 | - |
dc.language.iso | eng | - |
dc.publisher | Associação Brasileira de Divulgação Científica (ABRADIC) | - |
dc.source | Web of Science | - |
dc.subject | CDNA CLONING | pt |
dc.subject | DIFFERENTIAL HYBRIDIZATION | pt |
dc.subject | GLUCOCORTICOID-REGULATED GENES | pt |
dc.title | GLUCOCORTICOID-REGULATED GENE IN TRANSFORMED TO NORMAL PHENOTYPIC REVERSION | en |
dc.type | outro | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | UNIV SAO PAULO,INST QUIM,DEPT BIOQUIM,BR-01498970 SAO PAULO,SP,BRAZIL | - |
dc.description.affiliation | UNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,BR-14801360 ARARAQUARA,SP,BRAZIL | - |
dc.description.affiliationUnesp | UNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,BR-14801360 ARARAQUARA,SP,BRAZIL | - |
dc.identifier.wos | WOS:A1994MX60900067 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Brazilian Journal of Medical and Biological Research | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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