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dc.contributor.authorTaboga, S. R.-
dc.contributor.authorMello, M. L. S.-
dc.contributor.authorFalco, J. R. P.-
dc.contributor.authorVidal, B. C.-
dc.identifier.citationActa Histochemica Et Cytochemica. Kyoto: Japan Soc Histochem Cytochem, v. 29, n. 2, p. 129-134, 1996.-
dc.description.abstractThe pattern of availability of free DNA phosphates, and the kind of DNA-protein complex arrangement, both induced by nuclear basic proteins, and the richness in arginine residues in these proteins were investigated cytochemically and cytophysically in spermatozoa of the South-American Hylidae species, Hyla fuscovaria and Hyla biobeba. The aim was to demonstrate differences at the level of sperm histones in two species of Hyla until recently considered to be congeneric. The results indicated differences in the spermatozoal nuclear basic proteins and DNA-protein complexes when the two species were compared. The spermatozoa of Hyla biobeba were assumed to be likely to contain a Bloch's ''type 3'' protein type (intermediate sperm basic protein), similarly to Hyla species of North and Central America. on the other hand, the data obtained for the spermatozoa of Hyla fuscovaria indicated that they contain a protamine or protamine-like protein, differing from Hyla biobeba and Hyla species of North and Central America. It is suggested that the differences reported here may be genus-specific, since Hyla fuscovaria has recently been reclassified as Scinax fuscovaria based on parameters other than sperm histone types. These findings are in agreement with the general view of a wide variability in sperm nuclear proteins in the Anura group.en
dc.publisherJapan Soc Histochem Cytochem-
dc.sourceWeb of Science-
dc.subjectnuclear proteinspt
dc.subjectpolarization microscopypt
dc.titleCytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein compositionen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNICAMP,DEPT CELL BIOL,INST BIOL,BR-13083970 CAMPINAS,SP,BRAZIL-
dc.description.affiliationUNESP,IBILCE,DEPT BIOL,BR-15054000 S JOSE RIO PR,SP,BRAZIL-
dc.description.affiliationUnespUNESP,IBILCE,DEPT BIOL,BR-15054000 S JOSE RIO PR,SP,BRAZIL-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofActa Histochemica et Cytochemica-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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