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http://acervodigital.unesp.br/handle/11449/34591Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Gomes, E. | - |
| dc.contributor.author | Serzedello, A. | - |
| dc.date.accessioned | 2014-05-20T15:23:54Z | - |
| dc.date.accessioned | 2016-10-25T17:57:57Z | - |
| dc.date.available | 2014-05-20T15:23:54Z | - |
| dc.date.available | 2016-10-25T17:57:57Z | - |
| dc.date.issued | 1996-01-01 | - |
| dc.identifier | http://cat.inist.fr/?aModele=afficheN&cpsidt=2559335 | - |
| dc.identifier.citation | Microbios. Cambridge: Faculty Press, v. 87, n. 353, p. 227-237, 1996. | - |
| dc.identifier.issn | 0026-2633 | - |
| dc.identifier.uri | http://hdl.handle.net/11449/34591 | - |
| dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/34591 | - |
| dc.description.abstract | A ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55 degrees C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3'-nucleotides from yeast RNA. | en |
| dc.format.extent | 227-237 | - |
| dc.language.iso | eng | - |
| dc.publisher | Faculty Press | - |
| dc.source | Web of Science | - |
| dc.subject | ribonuclease | pt |
| dc.subject | Aspergillus | pt |
| dc.subject | nucleotides | pt |
| dc.title | Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes | en |
| dc.type | outro | - |
| dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
| dc.description.affiliation | UNIV ESTADUAL PAULISTA,INST BIOCENCIAS,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL | - |
| dc.description.affiliationUnesp | UNIV ESTADUAL PAULISTA,INST BIOCENCIAS,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL | - |
| dc.identifier.wos | WOS:A1996WJ24300003 | - |
| dc.rights.accessRights | Acesso restrito | - |
| dc.relation.ispartof | Microbios | - |
| Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp | |
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