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DC Field | Value | Language |
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dc.contributor.author | Milagres, B. G. | - |
dc.contributor.author | Neto, G. D. | - |
dc.contributor.author | Kubota, L. T. | - |
dc.contributor.author | Yamanaka, H. | - |
dc.date.accessioned | 2014-05-20T15:23:56Z | - |
dc.date.accessioned | 2016-10-25T17:57:59Z | - |
dc.date.available | 2014-05-20T15:23:56Z | - |
dc.date.available | 2016-10-25T17:57:59Z | - |
dc.date.issued | 1997-07-30 | - |
dc.identifier | http://dx.doi.org/10.1016/S0003-2670(97)00105-0 | - |
dc.identifier.citation | Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 347, n. 1-2, p. 35-41, 1997. | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/11449/34613 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/34613 | - |
dc.description.abstract | An amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10(-5) and 1.0x10(-4) mol l(-1) and the equation adjusted for this curve was I=(-0.04+/-0.01)+(11.4+/-0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method. | en |
dc.format.extent | 35-41 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | salicylate biosensor | pt |
dc.subject | polypyrrole film | pt |
dc.subject | salicylate hydroxylase | pt |
dc.subject | amperometry | pt |
dc.title | A new amperometric biosensor for salicylate based on salicylate hydroxylase immobilized on polipyrrole film doped with hexacyanoferrate | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | UNICAMP,INST QUIM,BR-13083970 CAMPINAS,SP,BRAZIL | - |
dc.description.affiliation | UNESP,INST QUIM,ARARAQUARA,SP,BRAZIL | - |
dc.description.affiliationUnesp | UNESP,INST QUIM,ARARAQUARA,SP,BRAZIL | - |
dc.identifier.doi | 10.1016/S0003-2670(97)00105-0 | - |
dc.identifier.wos | WOS:A1997XQ51300006 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Analytica Chimica Acta | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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