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http://acervodigital.unesp.br/handle/11449/36955
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DC Field | Value | Language |
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dc.contributor.author | Anjos, A. R. | - |
dc.contributor.author | Calvi, S. A. | - |
dc.contributor.author | Ferracini, R. | - |
dc.contributor.author | Peracoli, MTS | - |
dc.contributor.author | Silva, C. L. | - |
dc.contributor.author | Soares, AMVC | - |
dc.date.accessioned | 2014-05-20T15:26:53Z | - |
dc.date.accessioned | 2016-10-25T18:01:33Z | - |
dc.date.available | 2014-05-20T15:26:53Z | - |
dc.date.available | 2016-10-25T18:01:33Z | - |
dc.date.issued | 2002-08-01 | - |
dc.identifier | http://www.ingentaconnect.com/content/apl/mmy/2002/00000040/00000004/art00003 | - |
dc.identifier.citation | Medical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 40, n. 4, p. 377-382, 2002. | - |
dc.identifier.issn | 1369-3786 | - |
dc.identifier.uri | http://hdl.handle.net/11449/36955 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/36955 | - |
dc.description.abstract | The polysaccharide fraction of Paracoccidioides brasiliensis mycelial cell wall (F1 fraction), the active component of which is composed of beta-glucan, was investigated in regard to the activation of human monocytes for fungal killing. The cells were primed with interferon-gamma (IFN-gamma) or F1 (100 and 200 mug ml(-1)) or F1 (100 and 200 mug ml(-1)) plus IFN-gamma for 24 h and then evaluated for H2O2 release. In other experiments, the cells were pretreated with the same stimuli, challenged with a virulent strain of P. brasiliensis and evaluated for fungicidal activity and levels of tumor necrosis factor (TNF-alpha) in the supernatants. F1 increased the levels of H2O2 in a similar manner to IFN-gamma. However, a synergistic effect between these two activators was not detected. on the contrary, a significant fungicidal activity was only obtained after priming with IFN-gamma plus F1. This higher activity was associated with high levels of TNF-alpha in the supernatants of the cocultures. Overall, P. brasiliensis F1 fraction induced human monocytes to release relatively high levels of TNF-alpha, which, in combination with IFN-gamma, is responsible for the activation of human monocytes for effective killing of P. brasiliensis. | en |
dc.format.extent | 377-382 | - |
dc.language.iso | eng | - |
dc.publisher | B I O S Scientific Publishers Ltd | - |
dc.source | Web of Science | - |
dc.subject | beta-glucan | pt |
dc.subject | fungicidal activity | pt |
dc.subject | monocyte | pt |
dc.subject | Paracoccidioides brasiliensis | pt |
dc.subject | TNF | pt |
dc.title | Role of Paracoccidioides brasiliensis cell wall fraction containing beta-glucan in tumor necrosis factor-alpha production by human monocytes: correlation with fungicidal activity | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.description.affiliation | São Paulo State Univ, Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil | - |
dc.description.affiliation | Univ São Paulo, Sch Med, Dept Parasitol Microbiol & Immunol, BR-05508 São Paulo, Brazil | - |
dc.description.affiliationUnesp | São Paulo State Univ, Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil | - |
dc.identifier.doi | 10.1080/714031128 | - |
dc.identifier.wos | WOS:000177706100004 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Medical Mycology | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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