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dc.contributor.authorFonseca, F. V.-
dc.contributor.authorBaldissera, L.-
dc.contributor.authorCamargo, E. A. de-
dc.contributor.authorAntunes, E.-
dc.contributor.authorDiz-Filho, E. B. S.-
dc.contributor.authorCorrea, A. G.-
dc.contributor.authorBeriam, L. O. S.-
dc.contributor.authorToyama, D. O.-
dc.contributor.authorCotrim, C. A.-
dc.contributor.authorToyama, M. H.-
dc.date.accessioned2014-05-20T13:12:24Z-
dc.date.accessioned2016-10-25T16:32:53Z-
dc.date.available2014-05-20T13:12:24Z-
dc.date.available2016-10-25T16:32:53Z-
dc.date.issued2010-07-01-
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2010.03.004-
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 55, n. 8, p. 1527-1530, 2010.-
dc.identifier.issn0041-0101-
dc.identifier.urihttp://hdl.handle.net/11449/370-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/370-
dc.description.abstractWe show that ethyl 2-oxo-2H-chromene-3-carboxylate (EOCC), a synthetic coumarin, irreversibly inhibits phospholipase A(2) (sPLA2) from Crotalus durissus ruruima venom (sPLA2r) with an IC(50) of 3.1 +/- 0.06 nmol. EOCC strongly decreased the V(max) and K(m), and it virtually abolished the enzyme activity of sPLA2r as well as sPLA2s from other sources. The edema induced by 5PLA2r + EOCC was less than that induced by 5PLA2r treated with p-bromophenacyl bromide, which was more efficient at neutralizing the platelet aggregation activity of native 5PLA2r. Native 5PLA2r induced platelet aggregation of 91.54 +/- 9.3%, and sPLA2r +/- EOCC induced a platelet aggregation of 18.56 +/- 6.5%. EOCC treatment also decreased the myotoxic effect of sPLA2r. Mass spectrometry showed that EOCC formed a stable complex with sPLA2r, which increased the mass of native 5PLA2r from 14,299.34 da to 14,736.22 Da. Moreover, the formation of this complex appeared to be involved in the loss of 5PLA2r activity. Our results strongly suggest that EOCC can be used as a pharmacological agent against the 5PLA2 in Crotalus durissus sp. venom as well as other sPLA2s. (C) 2010 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.description.sponsorshipUNICAMP-
dc.description.sponsorshipUNESP-
dc.format.extent1527-1530-
dc.language.isoeng-
dc.publisherPergamon-Elsevier B.V. Ltd-
dc.sourceWeb of Science-
dc.subjectCrotalus durissus ruruimaen
dc.subjectEdemaen
dc.subjectMyonecrosisen
dc.subjectPlateleten
dc.subjectsPLA2en
dc.subjectSynthetic coumarinen
dc.titleEffect of the synthetic coumarin, ethyl 2-oxo-2H-chromene-3-carboxylate, on activity of Crotalus durissus ruruima sPLA2 as well as on edema and platelet aggregation induced by this factoren
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionCtr Expt-
dc.contributor.institutionUniv Presbiteriana Mackenzie-
dc.description.affiliationUNESP, BR-11330900 Sao Vicente, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Fac Med, Dept Pharmacol, Campinas, SP, Brazil-
dc.description.affiliationCtr Expt, Inst Biol, Lab Vegetal Bacteriol, São Paulo, Brazil-
dc.description.affiliationUniv Presbiteriana Mackenzie, CCBS, São Paulo, Brazil-
dc.description.affiliationUnespUNESP, BR-11330900 Sao Vicente, SP, Brazil-
dc.identifier.doi10.1016/j.toxicon.2010.03.004-
dc.identifier.wosWOS:000278674400012-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofToxicon-
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