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dc.contributor.authorVieira, D. F.-
dc.contributor.authorWatanabe, L.-
dc.contributor.authorSant'ana, C. D.-
dc.contributor.authorMarcussi, S.-
dc.contributor.authorSampaio, S. V.-
dc.contributor.authorSoares, A. M.-
dc.contributor.authorArni, R. K.-
dc.identifier.citationActa Biochimica Et Biophysica Sinica. Shanghai: Shanghai Inst Biochemistry, Academia Sinica, v. 36, n. 12, p. 798-802, 2004.-
dc.description.abstractA thrombin-like serine protease, jararassin-I, was isolated from the venom of Bothrops jararaca. The protein was obtained in high yield and purity by a single chromatographic step using the affinity resin Benzamidine-Sepharose CL-6B. SDS-PAGE and dynamic light scattering analyses indicated that the molecular mass of the enzyme was about 30 kD. The enzyme possessed fibrinogenolytic and coagulant activities. The jararassin-I degraded the Bbeta chain of fibrinogen while the Aalpha chain and gammachain were unchanged. Proteases inhibitors, PMSF and benzamidine inhibited the coagulant activity. These results showed jararassin-I is a serine protease similar to coagulating thrombin-like snake venom proteases, but it specifically cleaves Bbeta chain of bovine fibrinogen. Single crystals of enzyme were obtained (0.2 mmx0.2 mmx0.2 mm) and used for X-ray diffraction experiments.en
dc.publisherShanghai Inst Biochemistry, Academia Sinica-
dc.sourceWeb of Science-
dc.subjectsnake venompt
dc.subjectBothrops jararacapt
dc.subjectserine protease thrombin-likept
dc.subjectfibrinogenolytic activitypt
dc.titlePurification and characterization of jararassin-I, a thrombin-like enzyme from Bothrops jararaca snake venomen
dc.contributor.institutionUniv Ribeirao Preto-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationUniv Ribeirao Preto, Unidade Biotecnol, Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exactas, Dept Fis, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, FMRP, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exactas, Dept Fis, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofActa Biochimica et Biophysica Sinica-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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