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DC Field | Value | Language |
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dc.contributor.author | Do Amaral, S. H. | - |
dc.contributor.author | De Assis, S. A. | - |
dc.contributor.author | Oliveira, OMMD | - |
dc.date.accessioned | 2014-05-20T15:27:18Z | - |
dc.date.accessioned | 2016-10-25T18:02:06Z | - |
dc.date.available | 2014-05-20T15:27:18Z | - |
dc.date.available | 2016-10-25T18:02:06Z | - |
dc.date.issued | 2005-08-01 | - |
dc.identifier | http://dx.doi.org/10.1111/j.1745-4514.2005.00036.x | - |
dc.identifier.citation | Journal of Food Biochemistry. Oxford: Blackwell Publishing, v. 29, n. 4, p. 367-380, 2005. | - |
dc.identifier.issn | 0145-8884 | - |
dc.identifier.uri | http://hdl.handle.net/11449/37313 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/37313 | - |
dc.description.abstract | The enzyme pectin methylesterase (PME) from orange was extracted and partially purified by filtration on Sephadex G-100. The extraction buffer for orange PME was borate-acetate containing 0.4 M NaCl. Orange PME showed optimum pH at 8.0 and optimum temperature at 50C. The PME enzyme was completely inactivated after 1 min of incubation at 90C. The specific activity increased in the presence of 0.15 M NaCl or 0.025 M Na2SO4, 0.10 M KCl, 0.025 M K2SO4, 0.05 and 0.1 M NH4Cl. Lithium chloride and Li(2)SO(4)inhibited the enzymatic activity at all concentrations studied. The K-m and V(max)value of PME were 0.36 mg/mL and 5.26 mu mol/mL-mg protein, respectively. | en |
dc.format.extent | 367-380 | - |
dc.language.iso | eng | - |
dc.publisher | Blackwell Publishing | - |
dc.source | Web of Science | - |
dc.title | Partial purification and characterization of pectin methylesterase from orange (Citrus sinensis) cv. Pera-rio | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ Estadual Feira Santana | - |
dc.description.affiliation | UNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Feira Santana, Dept Saude, BR-44031460 Feira de Santana, BA, Brazil | - |
dc.description.affiliationUnesp | UNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil | - |
dc.identifier.doi | 10.1111/j.1745-4514.2005.00036.x | - |
dc.identifier.wos | WOS:000230728100003 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of Food Biochemistry | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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