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dc.contributor.authorAlves-Prado, Heloiza Ferreira-
dc.contributor.authorGomes, Eleni-
dc.contributor.authorda Silva, Roberto-
dc.identifier.citationApplied Biochemistry and Biotechnology. Totowa: Humana Press Inc., v. 137, p. 41-55, 2007.-
dc.description.abstractA cyclomaltodextrin glucanotransferase (E.C. from a newly isolated alkalophilic and moderately thermophilic Paenibacillus campinasensis strain H69-3 was purified as a homogeneous protein from culture supernatant. Cyclomaltodextrin glucanotransferase was produced during submerged fermentation at 45 degrees C and purified by gel filtration on Sephadex G50 ion exchange using a Q-Sepharose column and ion exchange using a Mono-Q column. The molecular weight of the purified enzyme was 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the pI was 5.3. The optimum pH for enzyme activity was 6.5, and it was stable in the pH range 6.0-11.5. The optimum temperature was 65 degrees C at pH 6.5, and it was thermally stable up to 60 degrees C without substrate during 1 h in the presence of 10 mm CaCl2. The enzyme activity increased in the presence of Co2+, Ba2+, and Mn2+. Using maltodextrin as substrate, the K-m and K-cat were 1.65 mg/mL and 347.9 mu mol/mg.min, respectively.en
dc.publisherHumana Press Inc-
dc.sourceWeb of Science-
dc.subjectCGTase characterizationpt
dc.subjectCGTase purificationpt
dc.subjectcyclomaltodextrin glucanotransferasept
dc.subjectthermostable CGTasept
dc.titlePurification and characterization of a cyclomaltodextrin glucanotransferase from Paenibacillus campinasensis strain H69-3en
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNESP, Biochem & Appl Microbiol Lab, Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUNESP, Rio Claro, SP, Brazil-
dc.description.affiliationUnespUNESP, Biochem & Appl Microbiol Lab, Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUnespUNESP, Rio Claro, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofApplied Biochemistry and Biotechnology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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