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dc.contributor.authorWasko, A. P.-
dc.contributor.authorMartins, C.-
dc.contributor.authorWright, J. M.-
dc.contributor.authorGaletti, P. M.-
dc.date.accessioned2014-05-20T15:28:43Z-
dc.date.accessioned2016-10-25T18:03:52Z-
dc.date.available2014-05-20T15:28:43Z-
dc.date.available2016-10-25T18:03:52Z-
dc.date.issued2001-10-01-
dc.identifierhttp://dx.doi.org/10.1139/gen-44-5-893-
dc.identifier.citationGenome. Ottawa: Natl Research Council Canada, v. 44, n. 5, p. 893-902, 2001.-
dc.identifier.issn0831-2796-
dc.identifier.urihttp://hdl.handle.net/11449/38480-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/38480-
dc.description.abstractThere are few reports on the genomic organization of 5S rDNA in fish species. To characterize the 5S rDNA nucleotide sequence and chromosomal localization in the Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA genes were highly conserved, while the NTSs were widely variable among the species analyzed. Moreover, two classes of NTS were detected in each species, characterized by base substitutions and insertions-deletions. Using fluorescence in situ hybridization (FISH), two 5S rDNA chromosome loci that could be related to the two 5S rDNA NTS classes were observed in at least one of the species studied. 5S rDNA sequencing and chromosomal localization permitted the characterization of Brycon spp. and suggest a higher similarity among some of them. The data obtained indicate that the 5S rDNA can be an useful genetic marker for species identification and evolutionary studies.en
dc.format.extent893-902-
dc.language.isoeng-
dc.publisherNatl Research Council Canada-
dc.sourceWeb of Science-
dc.subjectBryconpt
dc.subjectFISHpt
dc.subjectnontranscribed spacerpt
dc.subjectnucleotide sequencept
dc.subject5S rDNApt
dc.titleMolecular organization of 5S rDNA in fishes of the genus Bryconen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionDalhousie Univ-
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)-
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil-
dc.description.affiliationDalhousie Univ, Dept Biol, Halifax, NS B3H 4H6, Canada-
dc.description.affiliationUniv Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil-
dc.identifier.doi10.1139/gen-44-5-893-
dc.identifier.wosWOS:000171015300018-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofGenome-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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