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DC Field | Value | Language |
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dc.contributor.author | de Assis, Sandra Aparecida | - |
dc.contributor.author | Martins, Antonio Baldo Geraldo | - |
dc.contributor.author | de Faria Oliveira, Olga Maria Mascarenhas | - |
dc.date.accessioned | 2014-05-20T15:28:46Z | - |
dc.date.accessioned | 2016-10-25T18:03:57Z | - |
dc.date.available | 2014-05-20T15:28:46Z | - |
dc.date.available | 2016-10-25T18:03:57Z | - |
dc.date.issued | 2007-08-15 | - |
dc.identifier | http://dx.doi.org/10.1002/jsfa.2884 | - |
dc.identifier.citation | Journal of the Science of Food and Agriculture. Chichester: John Wiley & Sons Ltd, v. 87, n. 10, p. 1845-1849, 2007. | - |
dc.identifier.issn | 0022-5142 | - |
dc.identifier.uri | http://hdl.handle.net/11449/38525 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/38525 | - |
dc.description.abstract | The enzyme pectinmethylesterase (PME) from acerola was extracted and purified by gel anion-exchange chromatography (Q Sepharose) and filtration on Sephadex G-100. The results showed two different PME isoforms (PME1 and PME2), with molecular masses of 25.10 and 5.20 kDa, respectively. PMEI specific activity increased by 9.63% after 60 min incubation at 98 degrees C, while PME2 retained 66% of its specific activity under the same conditions. The K-m values of PMEI, PME2 and concentrated PME were 0.94, 0.08 and 0.08mg mL(-1), respectively. The V-max value of PMEI, PME2 and concentrated were 204.08, 2, 158.73 and 2.92 mu mol min(-1) mg(-1) protein, respectively. (c) 2007 Society of Chemical Industry. | en |
dc.format.extent | 1845-1849 | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-Blackwell | - |
dc.source | Web of Science | - |
dc.subject | pectinmethylesterase | pt |
dc.subject | acerola | pt |
dc.subject | kinetic characterization | pt |
dc.subject | purification | pt |
dc.subject | isoenzymes | pt |
dc.subject | heat stability | pt |
dc.title | Purification and characterization of pectin methylesterase from acerola (Malpighia glabra L.) | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ Estadual Feira de Santana | - |
dc.description.affiliation | UNESP, Dept Bioquim & Tecnol Quim, Inst Quim, BR-14801970 Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Feira de Santana, Dept Saude, BR-44031460 Feira de Santana, BA, Brazil | - |
dc.description.affiliation | UNESP, Dept Hort, Jaboticabal, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, Dept Bioquim & Tecnol Quim, Inst Quim, BR-14801970 Araraquara, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, Dept Hort, Jaboticabal, SP, Brazil | - |
dc.identifier.doi | 10.1002/jsfa.2884 | - |
dc.identifier.wos | WOS:000248240800008 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of the Science of Food and Agriculture | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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