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dc.contributor.authorAlves, Renata Tome-
dc.contributor.authorPovoa, Marinete Marins-
dc.contributor.authorGoldman, Ira F.-
dc.contributor.authorCavasini, Carlos Eugnio-
dc.contributor.authorBaptista Rossit, Andrea Regina-
dc.contributor.authorDantas Machado, Ricardo Luiz-
dc.identifier.citationDiagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 59, n. 4, p. 415-419, 2007.-
dc.description.abstractFor the molecular diagnosis of Plasmodium vivax variants (VK210, VK247, and P. vivax-like) using DNA amplification procedures in the laboratory, the choice of rapid and inexpensive identification products of the 3 different genotypes is an important prerequisite. We report here the standardization of a new polymerase chain reaction/restriction fragment length polymorphism technique to identify the 3 described P. vivax circumsporozoite protein (CSP) variants using amplification of the central immunodominant region of the CSP gene of this protozoan. The simplicity, specificity, and sensitivity of the system described here is important to determine the prevalence and the distribution of infection with these P. vivax genotypes in endemic and nonendemic malaria areas, enabling a better understanding of their phylogeny. (c) 2007 Published by Elsevier B.V.en
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectPlasmodium vivax genotypespt
dc.subjectcircumsporozoite proteinpt
dc.subjectPlasmodium vivax-likept
dc.titleA new polymerase chain reaction/restriction fragment length polymorphism protocol for Plasmodium vivax circumsporozoite protein genotype (VK210, VK247, and P-vivax-like) determinationen
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionMS SVS-
dc.contributor.institutionCtr Dis Control & Prevent-
dc.description.affiliationFac Med Sao Jose do Rio Preto, Dept Doencas Dermatol Infecciosas & Parasitarias, Ctr Invest Microorganisms, BR-15090000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationMS SVS, Inst Evandro Chagas, BR-67030000 Ananindeua, Para, Brazil-
dc.description.affiliationCtr Dis Control & Prevent, Atlanta, GA 30333 USA-
dc.description.affiliationFdn Fac Med Sao Jose do Rio Preto, FUNFARME, BR-15090000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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