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dc.contributor.authorde Sousa, RLM-
dc.contributor.authorMontassier, H. J.-
dc.contributor.authorPinto, A. A.-
dc.date.accessioned2014-05-20T15:30:04Z-
dc.date.accessioned2016-10-25T18:05:27Z-
dc.date.available2014-05-20T15:30:04Z-
dc.date.available2016-10-25T18:05:27Z-
dc.date.issued2000-11-01-
dc.identifierhttp://dx.doi.org/10.1128/CDLI.7.6.940-944.2000-
dc.identifier.citationClinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 7, n. 6, p. 940-944, 2000.-
dc.identifier.issn1071-412X-
dc.identifier.urihttp://hdl.handle.net/11449/39511-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/39511-
dc.description.abstractA liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutination-inhibition (HI) test was regarded as the reference method, the cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r = 0.875). The two tests showed good agreement (<kappa> = 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.en
dc.format.extent940-944-
dc.language.isoeng-
dc.publisherAmer Soc Microbiology-
dc.sourceWeb of Science-
dc.titleDetection and quantification of antibodies to Newcastle disease virus in ostrich and rhea sera using a liquid phase blocking enzyme-linked immunosorbent assayen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionFdn Amparo Pesquisa Estado-
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agrarias & Vet, Dept Vet Pathol, Lab Virol & Immunol, BR-14870000 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Grad Program Microbiol, BR-05508 São Paulo, Brazil-
dc.description.affiliationFdn Amparo Pesquisa Estado, São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agrarias & Vet, Dept Vet Pathol, Lab Virol & Immunol, BR-14870000 São Paulo, Brazil-
dc.identifier.doi10.1128/CDLI.7.6.940-944.2000-
dc.identifier.wosWOS:000167743800016-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileWOS000167743800016.pdf-
dc.relation.ispartofClinical and Diagnostic Laboratory Immunology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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