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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/40244
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dc.contributor.authorPedrolli, D. B.-
dc.contributor.authorCarmona, E. C.-
dc.date.accessioned2014-05-20T15:30:58Z-
dc.date.accessioned2016-10-25T18:06:41Z-
dc.date.available2014-05-20T15:30:58Z-
dc.date.available2016-10-25T18:06:41Z-
dc.date.issued2009-11-01-
dc.identifierhttp://dx.doi.org/10.1134/S0003683809060064-
dc.identifier.citationApplied Biochemistry and Microbiology. New York: Maik Nauka/interperiodica/springer, v. 45, n. 6, p. 610-616, 2009.-
dc.identifier.issn0003-6838-
dc.identifier.urihttp://hdl.handle.net/11449/40244-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/40244-
dc.description.abstractThe aim of this study was to investigate some of the factors affecting pectin lyase (PL) production by an Aspergillus giganteus strain, and to characterize this pectinolytic activity excreted into the medium. The highest activities were obtained with orange waste, citrus pectin and galacturonic acid as carbon sources. The highest activity, using citrus pectin as carbon source, was obtained in 11-day-old standing cultures, but the highest specific activity was obtained in 6.5-day-old shaken cultures, at pH 6.5 and 35A degrees C. Using orange waste as carbon source, the highest activity was observed in 8-day-old standing cultures, at pH 7.0 and 30A degrees C. Optimal assay conditions were pH 8.5-9.0 and 50A degrees C. The PL activity showed thermal stability, with half-lives of 30 and 27 min when incubated at 45 and 50A degrees C, respectively. High stability was observed at room temperature from pH 6.0 to 10.0; more than 85% of enzyme activity was preserved in this pH range. Under optimum conditions, the highest pectin lyase activity in the medium was 470 U/ml, with orange waste as carbon source.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipCPKelco-Limeira, SP, Brazil-
dc.format.extent610-616-
dc.language.isoeng-
dc.publisherMaik Nauka/interperiodica/springer-
dc.sourceWeb of Science-
dc.titlePectin lyase from Aspergillus giganteus: Comparative study of productivity of submerged fermentation on citrus pectin and orange wasteen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationSão Paulo State Univ, Biochemystry & Microbiol Dept, Dept Biosci, UNESP, BR-13506900 São Paulo, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Biochemystry & Microbiol Dept, Dept Biosci, UNESP, BR-13506900 São Paulo, Brazil-
dc.identifier.doi10.1134/S0003683809060064-
dc.identifier.wosWOS:000271527200006-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofApplied Biochemistry and Microbiology-
dc.identifier.orcid0000-0002-3034-6497pt
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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