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dc.contributor.authorCiapina, Luciane Prioli-
dc.contributor.authorPicchi, Simone Cristina-
dc.contributor.authorLacroix, Jean-Marie-
dc.contributor.authorGertrudes de Macedo Lemos, Eliana-
dc.contributor.authorOedberg-Ferragut, Carmen-
dc.identifier.citationCanadian Journal of Microbiology. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 57, n. 2, p. 149-154, 2011.-
dc.description.abstractThe twin-arginine translocation (Tat) pathway of the xylem-limited phytopathogenic bacterium Xylella fastidiosa strain 9a5c, responsible for citrus variegated chlorosis, was explored. The presence of tatA, tatB, and tatC in the X. fastidiosa genome together with a list of proteins harboring 2 consecutive arginines in their signal peptides suggested the presence of a Tat pathway. The functional Tat dependence of X. fastidiosa OpgD was examined. Native or mutated signal peptides were fused to the beta-lactamase. Expression of fusion with intact signal peptides mediated high resistance to ampicillin in Escherichia coli tat(+) but not in the E. coli tat null mutant. The replacement of the 2 arginines by 2 lysines prevented the export of b-lactamase in E. coli tat(+), demonstrating that X. fastidiosa OpgD carries a signal peptide capable of engaging the E. coli Tat machinery. RT-PCR analysis revealed that the tat genes are transcribed as a single operon. tatA, tatB, and tatC genes were cloned. Complementation assays in E. coli devoid of all Tat or TatC components were unsuccessful, whereas X. fastidiosa Tat components led to a functional Tat translocase in E. coli TatB-deficient strain. Additional experiments implicated that X. fastidiosa TatB component could form a functional heterologous complex with the E. coli TatC component.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.description.sponsorshipCentre national de la recherche scientifique (CNRS)-
dc.publisherCanadian Science Publishing, Nrc Research Press-
dc.sourceWeb of Science-
dc.subjectTat transporten
dc.subjectXylella fastidiosaen
dc.titleA putative twin-arginine translocation system in the phytopathogenic bacterium Xylella fastidiosaen
dc.contributor.institutionUniv Lille 1-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Lille 1, USTL CNRS, UMR 8576, Unite Glycobiol Struct & Fonctionnelle,IFR 147, F-59655 Villeneuve Dascq, France-
dc.description.affiliationUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil-
dc.description.sponsorshipIdCAPES: 370/01-
dc.description.sponsorshipIdCNRS: UMR 8576-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCanadian Journal of Microbiology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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