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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/41094
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dc.contributor.authorFeldman, Mark-
dc.contributor.authorVu Dang La,-
dc.contributor.authorLombardo Bedran, Telma Blanca-
dc.contributor.authorPalomari Spolidorio, Denise Madalena-
dc.contributor.authorGrenier, Daniel-
dc.date.accessioned2014-05-20T15:32:06Z-
dc.date.accessioned2016-10-25T18:08:13Z-
dc.date.available2014-05-20T15:32:06Z-
dc.date.available2016-10-25T18:08:13Z-
dc.date.issued2011-12-01-
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2011.07.009-
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 14-15, p. 1261-1269, 2011.-
dc.identifier.issn1286-4579-
dc.identifier.urihttp://hdl.handle.net/11449/41094-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/41094-
dc.description.abstractExtracellular matrix metalloproteinase inducer (EMMPRIN) or CD 147 is a transmembrane glycoprotein expressed by various cell types, including oral epithelial cells. Recent studies have brought evidence that EMMPRIN plays a role in periodontitis. In the present study, we investigated the effect of Porphyromonas gingivalis, a major pathogen in chronic periodontitis, on the shedding of membrane-anchored EMMPRIN and on the expression of the EMMPRIN gene by oral epithelial cells. A potential contribution of shed EMMPRIN to the inflammatory process of periodontitis was analyzed by evaluating the effect of recombinant EMMPRIN on cytokine and matrix metalloproteinase (MMP) secretion by human gingival fibroblasts. ELISA and immunofluorescence analyses revealed that P. gingivalis mediated the shedding of epithelial cell-surface EMMPRIN in a dose- and time-dependent manner. Cysteine proteinase (gingipain)-deficient P. gingivalis mutants were used to demonstrate that both Arg- and Lys-gingipain activities are involved in EMMPRIN shedding. Real-time PCR showed that P. gingivalis had no significant effect on the expression of the EMMPRIN gene in epithelial cells. Recombinant EMMPRIN induced the secretion of IL-6 and MMP-3 by gingival fibroblasts, a phenomenon that appears to involve mitogen activated protein kinases. The present study brought to light a new mechanism by which P. gingivalis can promote the inflammatory response during periodontitis. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.sponsorshipCanadian Institutes of Health Research (CIHR)-
dc.format.extent1261-1269-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectCytokineen
dc.subjectEMMPRINen
dc.subjectEpithelial cellen
dc.subjectFibroblasten
dc.subjectPeriodontitisen
dc.subjectPorphyromonas gingivalisen
dc.titlePorphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (EMMPRIN) by oral epithelial cells: a potential role in inflammatory periodontal diseaseen
dc.typeoutro-
dc.contributor.institutionUniv Laval-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Laval, Oral Ecol Res Grp, Fac Dent, Quebec City, PQ G1V 0A6, Canada-
dc.description.affiliationState Univ São Paulo, Araraquara Dent Sch, Araraquara, Brazil-
dc.description.affiliationUnespState Univ São Paulo, Araraquara Dent Sch, Araraquara, Brazil-
dc.identifier.doi10.1016/j.micinf.2011.07.009-
dc.identifier.wosWOS:000298127800015-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofMicrobes and Infection-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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