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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/41762
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dc.contributor.authorPaneto, Greiciane G.-
dc.contributor.authorKoehnemann, Stephan-
dc.contributor.authorMartins, Joyce A.-
dc.contributor.authorCicarelli, Regina Maria Barretto-
dc.contributor.authorPfeiffer, Heidi-
dc.date.accessioned2014-05-20T15:33:01Z-
dc.date.accessioned2016-10-25T18:09:26Z-
dc.date.available2014-05-20T15:33:01Z-
dc.date.available2016-10-25T18:09:26Z-
dc.date.issued2011-03-01-
dc.identifierhttp://dx.doi.org/10.1016/j.mito.2010.12.010-
dc.identifier.citationMitochondrion. Oxford: Elsevier B.V., v. 11, n. 2, p. 296-302, 2011.-
dc.identifier.issn1567-7249-
dc.identifier.urihttp://hdl.handle.net/11449/41762-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/41762-
dc.description.abstractSNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)-
dc.format.extent296-302-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectAdmixture populationsen
dc.subjectBrazilen
dc.subjectHaplogroup affiliationen
dc.subjectMitochondrial DNAen
dc.subjectSNaPshot reactionen
dc.titleA single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroupsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniv Munster-
dc.description.affiliationUnesp Univ Estadual Paulista, Fac Ciencias Farmaceut, Lab Invest Paternidade, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUniv Munster, Inst Legal Med, D-48149 Munster, Germany-
dc.description.affiliationUnespUnesp Univ Estadual Paulista, Fac Ciencias Farmaceut, Lab Invest Paternidade, BR-14801902 Araraquara, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 05/03984-5-
dc.description.sponsorshipIdCNPq: 2007-0/140052-
dc.description.sponsorshipIdDAAD: A0971734-
dc.identifier.doi10.1016/j.mito.2010.12.010-
dc.identifier.wosWOS:000287112100008-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofMitochondrion-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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