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http://acervodigital.unesp.br/handle/11449/42302
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DC Field | Value | Language |
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dc.contributor.author | Estofolete, Cassia F. | - |
dc.contributor.author | Zucoloto, Sergio | - |
dc.contributor.author | Oliani, Sonia M. | - |
dc.contributor.author | Polli-Lopes, Ana Claudia | - |
dc.contributor.author | Gil, Cristiane D. | - |
dc.date.accessioned | 2014-05-20T15:33:45Z | - |
dc.date.accessioned | 2016-10-25T18:10:27Z | - |
dc.date.available | 2014-05-20T15:33:45Z | - |
dc.date.available | 2016-10-25T18:10:27Z | - |
dc.date.issued | 2011-06-01 | - |
dc.identifier | http://dx.doi.org/10.1007/s10620-010-1516-7 | - |
dc.identifier.citation | Digestive Diseases and Sciences. Dordrecht: Springer, v. 56, n. 6, p. 1637-1644, 2011. | - |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.uri | http://hdl.handle.net/11449/42302 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/42302 | - |
dc.description.abstract | Background This study evaluated the galectin-1 and -3 expression during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in denervated rat stomachs using benzalkonium chloride.Method Four experimental situations were evaluated: nondenervated and denervated stomachs without lesions and nondenervated and denervated stomachs with lesions. Sections of the pyloric region were stained with toluidine blue and incubated with mouse monoclonal anti-Gal-1 and rabbit polyclonal anti-Gal-3 for histopathological and immunohistochemical analysis, respectively.Result MNNG caused the development of benign and malignant epithelial lesions, which were more pronounced in nondenervated stomachs with lesions and accompanied by inflammatory cell-enriched stroma. By immunostaining, the epithelial cells, blood vessels, muscle layer, and myenteric plexus were Gal-1 and -3 positive. Gal-3 was also detected in the gastric crypts, mucus secretion, and fibroblasts of pyloric fragments. Development of lesions in denervated stomachs was associated with a significant decrease in Gal-1 and -3 expression in epithelial cells, mast cells, and neutrophil cytoplasm, compared with that of nondenervated stomach lesions (P < 0.01; P < 0.001; P < 0.001, respectively).Conclusion These results demonstrate that myenteric denervation downregulates endogenous Gal-1 and -3 expression, which might inhibit tumor development in this experimental model. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 1637-1644 | - |
dc.language.iso | eng | - |
dc.publisher | Springer | - |
dc.source | Web of Science | - |
dc.subject | Benzalkonium chloride | en |
dc.subject | Galectin | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Mast cells | en |
dc.subject | MNNG | en |
dc.subject | Neutrophil | en |
dc.title | Myenteric Denervation Downregulates Galectin-1 and-3 Expression in Gastric Carcinogenesis | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | - |
dc.contributor.institution | Faculdade de Medicina de São José do Rio Preto (FAMERP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Fed São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil | - |
dc.description.affiliation | Sao Jose do Rio Preto Sch Med FAMERP, Dept Anat, BR-15090000 São Paulo, Brazil | - |
dc.description.affiliation | Ribeirao Preto Sch Med FMRP USP, Dept Pathol, BR-14049900 São Paulo, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, IBILCE, Dept Biol, BR-15054000 São Paulo, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, IBILCE, Dept Biol, BR-15054000 São Paulo, Brazil | - |
dc.description.sponsorshipId | FAPESP: 08/05722-6 | - |
dc.description.sponsorshipId | CNPq: 306074/2007-9 | - |
dc.identifier.doi | 10.1007/s10620-010-1516-7 | - |
dc.identifier.wos | WOS:000290770900009 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Digestive Diseases and Sciences | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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