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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/64022
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dc.contributor.authorda Silva, Elisete-
dc.contributor.authorLourenco, Euclides J.-
dc.contributor.authorNeves, Valdir Augusto-
dc.date.accessioned2014-05-27T09:49:59Z-
dc.date.accessioned2016-10-25T18:12:31Z-
dc.date.available2014-05-27T09:49:59Z-
dc.date.available2016-10-25T18:12:31Z-
dc.date.issued1990-12-01-
dc.identifierhttp://dx.doi.org/10.1016/0031-9422(90)85401-Z-
dc.identifier.citationPhytochemistry, v. 29, n. 4, p. 1051-1056, 1990.-
dc.identifier.issn0031-9422-
dc.identifier.urihttp://hdl.handle.net/11449/64022-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/64022-
dc.description.abstractSoluble and bound peroxidases were isolated from the pulp of ripening papaya fruit. During papaya ripening, soluble and bound peroxidase activities increased 2.5- and 4.2-fold, respectively. Soluble peroxidase was purified 59-fold by ammonium sulphate precipitation and chromatography on Sephadex G-25, DEAE-cellulose and Sephadex G-100. Bound peroxidase was purified 140-fold by ammonium sulphate precipitation and chromatography on Sephadex G-100 and DEAE-cellulose. Polyacrylamide gel electrophoresis of the purified preparations revealed that both enzymes were highly purified by the procedures adopted. The soluble and bound forms had a Mr of 41 000 and 54 000, respectively. Soluble and bound peroxidases showed optimum activity at pH 6.0 and 5.5, respectively, and were inhibited by p-chloromercuribenzoate, iodoacetamide, N-ethylmaleimide, potassium cyanide and Fe2+. Soluble peroxidase was activated by ammonium sulphate and this activation was prevented by cyanide. © 1990.en
dc.format.extent1051-1056-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCarica papaya-
dc.subjectCaricaceae-
dc.subjectenzyme properties.-
dc.subjectpapaya fruit-
dc.subjectperoxidase-
dc.subjectripening-
dc.titleSoluble and bound peroxidases from papaya fruiten
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Food Nutrition FCF UNESP, Brasil- Rodovia Araraquara, -Jau Km 1, 14800 Araraquara-
dc.description.affiliationUnespDepartment of Food Nutrition FCF UNESP, Brasil- Rodovia Araraquara, -Jau Km 1, 14800 Araraquara-
dc.identifier.doi10.1016/0031-9422(90)85401-Z-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofPhytochemistry-
dc.identifier.scopus2-s2.0-0001128831-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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