Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/64022
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | da Silva, Elisete | - |
dc.contributor.author | Lourenco, Euclides J. | - |
dc.contributor.author | Neves, Valdir Augusto | - |
dc.date.accessioned | 2014-05-27T09:49:59Z | - |
dc.date.accessioned | 2016-10-25T18:12:31Z | - |
dc.date.available | 2014-05-27T09:49:59Z | - |
dc.date.available | 2016-10-25T18:12:31Z | - |
dc.date.issued | 1990-12-01 | - |
dc.identifier | http://dx.doi.org/10.1016/0031-9422(90)85401-Z | - |
dc.identifier.citation | Phytochemistry, v. 29, n. 4, p. 1051-1056, 1990. | - |
dc.identifier.issn | 0031-9422 | - |
dc.identifier.uri | http://hdl.handle.net/11449/64022 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/64022 | - |
dc.description.abstract | Soluble and bound peroxidases were isolated from the pulp of ripening papaya fruit. During papaya ripening, soluble and bound peroxidase activities increased 2.5- and 4.2-fold, respectively. Soluble peroxidase was purified 59-fold by ammonium sulphate precipitation and chromatography on Sephadex G-25, DEAE-cellulose and Sephadex G-100. Bound peroxidase was purified 140-fold by ammonium sulphate precipitation and chromatography on Sephadex G-100 and DEAE-cellulose. Polyacrylamide gel electrophoresis of the purified preparations revealed that both enzymes were highly purified by the procedures adopted. The soluble and bound forms had a Mr of 41 000 and 54 000, respectively. Soluble and bound peroxidases showed optimum activity at pH 6.0 and 5.5, respectively, and were inhibited by p-chloromercuribenzoate, iodoacetamide, N-ethylmaleimide, potassium cyanide and Fe2+. Soluble peroxidase was activated by ammonium sulphate and this activation was prevented by cyanide. © 1990. | en |
dc.format.extent | 1051-1056 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | Carica papaya | - |
dc.subject | Caricaceae | - |
dc.subject | enzyme properties. | - |
dc.subject | papaya fruit | - |
dc.subject | peroxidase | - |
dc.subject | ripening | - |
dc.title | Soluble and bound peroxidases from papaya fruit | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Department of Food Nutrition FCF UNESP, Brasil- Rodovia Araraquara, -Jau Km 1, 14800 Araraquara | - |
dc.description.affiliationUnesp | Department of Food Nutrition FCF UNESP, Brasil- Rodovia Araraquara, -Jau Km 1, 14800 Araraquara | - |
dc.identifier.doi | 10.1016/0031-9422(90)85401-Z | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Phytochemistry | - |
dc.identifier.scopus | 2-s2.0-0001128831 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.