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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/64377
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dc.contributor.authorHossepian de Lima, V. F M-
dc.contributor.authorMoreira-Filho, C. A.-
dc.contributor.authorDe Bem, A. R.-
dc.contributor.authorJorge, W.-
dc.date.accessioned2014-05-27T11:17:53Z-
dc.date.accessioned2016-10-25T18:13:09Z-
dc.date.available2014-05-27T11:17:53Z-
dc.date.available2016-10-25T18:13:09Z-
dc.date.issued1993-06-01-
dc.identifierhttp://dx.doi.org/10.1016/0093-691X(93)90236-X-
dc.identifier.citationTheriogenology, v. 39, n. 6, p. 1343-1352, 1993.-
dc.identifier.issn0093-691X-
dc.identifier.urihttp://hdl.handle.net/11449/64377-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/64377-
dc.description.abstractMurine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993.en
dc.format.extent1343-1352-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectanti-H-Y antibodies-
dc.subjectembryo-
dc.subjectimmunological assays-
dc.subjectsexing-
dc.titleSex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assaysen
dc.typeoutro-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationEmbrapa-Cenargen, Caixa Postal 02372, CEP 70770 Brasília, DF-
dc.description.affiliationDepartamento de Imunologia ICB USP São Paulo, SP-
dc.description.affiliationDepartamento de Genética UNESP Botucatu, SP-
dc.description.affiliationUnespDepartamento de Genética UNESP Botucatu, SP-
dc.identifier.doi10.1016/0093-691X(93)90236-X-
dc.identifier.wosWOS:A1993LF25800013-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofTheriogenology-
dc.identifier.scopus2-s2.0-38249001637-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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