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http://acervodigital.unesp.br/handle/11449/64759
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DC Field | Value | Language |
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dc.contributor.author | Sandoval, M. P. | - |
dc.contributor.author | Del Negro, G. M. B. | - |
dc.contributor.author | Mendes-Giannini, Maria José Soares | - |
dc.contributor.author | Brito, T. de | - |
dc.date.accessioned | 2014-05-27T11:18:04Z | - |
dc.date.accessioned | 2016-10-25T18:13:49Z | - |
dc.date.available | 2014-05-27T11:18:04Z | - |
dc.date.available | 2016-10-25T18:13:49Z | - |
dc.date.issued | 1996-03-01 | - |
dc.identifier.citation | Journal de Mycologie Medicale, v. 6, n. 1, p. 1-6, 1996. | - |
dc.identifier.issn | 1156-5233 | - |
dc.identifier.uri | http://hdl.handle.net/11449/64759 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/64759 | - |
dc.description.abstract | Objective. Paracoccidioides brasiliensis antigens (strain 113) were located at ultrastructural level in both yeast and mycelial forms of the fungus. The reactivity of the sera employed was analysed. Materials and methods. Immunofluorescence and ultrastructural protein A-gold immunolabelling techniques were performed using two polyclonal antisera: one against P. brasiliensis exoantigens and the other against a 43-kDa glycoprotein (gp43). Immunoblotting assays were employed to define reactivity of these antisera with somatic and metabolic antigens of both forms of the fungus. Results. The techniques employed revealed in both yeast and mycelial forms of P. brasiliensis a similar antigenic distribution. The antigens deposits were seen within the cytoplasm, and over the cell wall of the fungus. The anti-exoantigen serum recognized several bands in both forms of the fungus. The anti-gp43 serum reacted strongly with the 43-kDa fraction and weakly with few other fractions. Conclusions. Immunocytochemical techniques suggest a protein synthesis within the cytoplasm followed by excretion through the cell wall. Similar results employing both polyclonal antisera were obtained. | en |
dc.format.extent | 1-6 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | antigens | - |
dc.subject | Paracoccidioides brasiliensis | - |
dc.subject | ultrastructural labeling | - |
dc.subject | glycoprotein | - |
dc.subject | polyclonal antibody | - |
dc.subject | antigen expression | - |
dc.subject | cell wall | - |
dc.subject | controlled study | - |
dc.subject | immunocytochemistry | - |
dc.subject | immunofluorescence | - |
dc.subject | nonhuman | - |
dc.subject | paracoccidioides brasiliensis | - |
dc.subject | protein synthesis | - |
dc.subject | ultrastructure | - |
dc.subject | yeast | - |
dc.title | Distribution of exoantigens and a 43-kDa glycoprotein (gp43) in the yeast and mycelial forms of Paracoccidioides brasiliensis | en |
dc.type | outro | - |
dc.contributor.institution | Institute of Tropical Medicine | - |
dc.contributor.institution | Medical School | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.description.affiliation | Department of Pathology Institute of Tropical Medicine | - |
dc.description.affiliation | Laboratory of Medical Mycology University of S. Paulo Medical School | - |
dc.description.affiliation | Department of Clinical Analysis Faculty of Pharmaceutical Sciences UNESP, S. Paulo | - |
dc.description.affiliation | Faculdade de Medicina da USP Departamento de Patologia, Av. Dr Arnaldo, 455, 01246 903, S. Paulo, S.P. | - |
dc.description.affiliationUnesp | Department of Clinical Analysis Faculty of Pharmaceutical Sciences UNESP, S. Paulo | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal de Mycologie Medicale | - |
dc.identifier.scopus | 2-s2.0-0029797985 | - |
dc.identifier.orcid | 0000-0002-8059-0826 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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