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Cloning and expression of the cDNA encoding rat granulocyte colony-stimulating factor
  • University of Washington
  • Universidade Estadual Paulista (UNESP)
Granulocyte colony-stimulating factor (G-CSF) acts on precursor hematopoietic cells to control the production and maintenance of neutrophils. Recombinant G-CSF (re-G-CSF)is used clinically to treat patients with neutropenia and has greatly reduced the infection risk associated with bone marrow transplantation. Cyclic hematopoiesis, a stem cell defect characterized by severe recurrent neutropenia, occurs in man and grey collie dogs, and can be treated by administration of re-G-CSF. Availability of the rat G-CSF cDNA would benefit the use of rats as models of gene therapy for the treatment of cyclic hematopoiesis. In preliminary rat experiments, retroviral-mediated expression of canine G-CSF caused neutralizing antibody formation which precluded long-term increases in neutrophil counts. To overcome this problem we cloned the rat G-CSF cDNA from RNA isolated from skin fibroblasts. The rat G-CSF sequence shared a high degree of identity in both the coding and non-coding regions with both the murine G-CSF (85%) and human G-CSF (74%). The signal peptides of murine and human G-CSF both contained 30 amino acids (aa), whereas the deduced signal sequence for rat G-CSF possessed 21 aa. A retrovirus encoding the rat G-CSF cDNA synthesized bioactive G-CSF from transduced vascular smooth muscle cells.
Issue Date: 
Gene, v. 175, n. 1-2, p. 101-104, 1996.
Time Duration: 
  • Gene therapy
  • Neutropenia
  • Nucleotide sequence
  • complementary dna
  • granulocyte colony stimulating factor
  • neutralizing antibody
  • recombinant granulocyte colony stimulating factor
  • animal cell
  • animal experiment
  • animal model
  • bone marrow transplantation
  • dog
  • gene therapy
  • graft infection
  • hematopoiesis
  • hematopoietic cell
  • human
  • human cell
  • molecular cloning
  • neutropenia
  • neutrophil
  • nonhuman
  • priority journal
  • rat
  • retrovirus
  • stem cell
  • vascular smooth muscle
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Genetic Vectors
  • Granulocyte Colony-Stimulating Factor
  • Mice
  • Molecular Sequence Data
  • Rats
  • Sequence Analysis, DNA
  • Sequence Homology
  • Animalia
  • Canis familiaris
  • Murinae
  • unidentified retrovirus
Access Rights: 
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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