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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/64860
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dc.contributor.authorHan, Sang Won-
dc.contributor.authorRamesh, N.-
dc.contributor.authorOsborne, William R.A.-
dc.date.accessioned2014-05-27T11:18:07Z-
dc.date.accessioned2016-10-25T18:14:00Z-
dc.date.available2014-05-27T11:18:07Z-
dc.date.available2016-10-25T18:14:00Z-
dc.date.issued1996-10-10-
dc.identifierhttp://dx.doi.org/10.1016/0378-1119(96)00131-X-
dc.identifier.citationGene, v. 175, n. 1-2, p. 101-104, 1996.-
dc.identifier.issn0378-1119-
dc.identifier.urihttp://hdl.handle.net/11449/64860-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/64860-
dc.description.abstractGranulocyte colony-stimulating factor (G-CSF) acts on precursor hematopoietic cells to control the production and maintenance of neutrophils. Recombinant G-CSF (re-G-CSF)is used clinically to treat patients with neutropenia and has greatly reduced the infection risk associated with bone marrow transplantation. Cyclic hematopoiesis, a stem cell defect characterized by severe recurrent neutropenia, occurs in man and grey collie dogs, and can be treated by administration of re-G-CSF. Availability of the rat G-CSF cDNA would benefit the use of rats as models of gene therapy for the treatment of cyclic hematopoiesis. In preliminary rat experiments, retroviral-mediated expression of canine G-CSF caused neutralizing antibody formation which precluded long-term increases in neutrophil counts. To overcome this problem we cloned the rat G-CSF cDNA from RNA isolated from skin fibroblasts. The rat G-CSF sequence shared a high degree of identity in both the coding and non-coding regions with both the murine G-CSF (85%) and human G-CSF (74%). The signal peptides of murine and human G-CSF both contained 30 amino acids (aa), whereas the deduced signal sequence for rat G-CSF possessed 21 aa. A retrovirus encoding the rat G-CSF cDNA synthesized bioactive G-CSF from transduced vascular smooth muscle cells.en
dc.format.extent101-104-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectGene therapy-
dc.subjectNeutropenia-
dc.subjectNucleotide sequence-
dc.subjectcomplementary dna-
dc.subjectgranulocyte colony stimulating factor-
dc.subjectneutralizing antibody-
dc.subjectrecombinant granulocyte colony stimulating factor-
dc.subjectanimal cell-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectbone marrow transplantation-
dc.subjectdog-
dc.subjectgene therapy-
dc.subjectgraft infection-
dc.subjecthematopoiesis-
dc.subjecthematopoietic cell-
dc.subjecthuman-
dc.subjecthuman cell-
dc.subjectmolecular cloning-
dc.subjectneutropenia-
dc.subjectneutrophil-
dc.subjectnonhuman-
dc.subjectpriority journal-
dc.subjectrat-
dc.subjectretrovirus-
dc.subjectstem cell-
dc.subjectvascular smooth muscle-
dc.subjectAmino Acid Sequence-
dc.subjectAnimals-
dc.subjectBase Sequence-
dc.subjectCloning, Molecular-
dc.subjectDNA, Complementary-
dc.subjectGenetic Vectors-
dc.subjectGranulocyte Colony-Stimulating Factor-
dc.subjectMice-
dc.subjectMolecular Sequence Data-
dc.subjectRats-
dc.subjectSequence Analysis, DNA-
dc.subjectSequence Homology-
dc.subjectAnimalia-
dc.subjectCanis familiaris-
dc.subjectMurinae-
dc.subjectunidentified retrovirus-
dc.titleCloning and expression of the cDNA encoding rat granulocyte colony-stimulating factoren
dc.typeoutro-
dc.contributor.institutionUniversity of Washington-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Pediatrics MS 356320 University of Washington, Seattle, WA 98195-
dc.description.affiliationDepartment of Biochemistry-IB Unesp Rio Claro, Sao Paulo 13500-
dc.description.affiliationUnespDepartment of Biochemistry-IB Unesp Rio Claro, Sao Paulo 13500-
dc.identifier.doi10.1016/0378-1119(96)00131-X-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofGene-
dc.identifier.scopus2-s2.0-0030579145-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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