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DC Field | Value | Language |
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dc.contributor.author | Fernandes, João Roberto | - |
dc.contributor.author | De Oliveira Neto, Graciliano | - |
dc.contributor.author | Kubota, Lauro T. | - |
dc.contributor.author | Tubino, Matthieu | - |
dc.date.accessioned | 2014-05-27T11:18:07Z | - |
dc.date.accessioned | 2016-10-25T18:14:01Z | - |
dc.date.available | 2014-05-27T11:18:07Z | - |
dc.date.available | 2016-10-25T18:14:01Z | - |
dc.date.issued | 1996-11-01 | - |
dc.identifier | http://dx.doi.org/10.1039/AC9963300397 | - |
dc.identifier.citation | Analytical Communications, v. 33, n. 11, p. 397-399, 1996. | - |
dc.identifier.issn | 1359-7337 | - |
dc.identifier.uri | http://hdl.handle.net/11449/64871 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/64871 | - |
dc.description.abstract | The enzyme oxalate oxidase, E.C. 1.2.3.4 from Sorghum vulgare seeds (variety BR303) was used to develop a new sensor for oxalate determination without any purification. The sorghum seeds were conditioned in a 0.10 mol I-1 KCl solution. Then, these seeds were put in a stirring bar type enzymic reactor and coupled with an electrode for CO2. This device was introduced into a cell containing 10.0 ml of a 0.10 mol I-1 KCl solution saturated with oxygen. This sensor showed a linear response between 1.0 and 4.0 × 10-3 mol I-1 with a slope of 30 mV per decade of oxalate concentration at 25.0°C. The sensor was stable for one month or 200 determinations. The response time was about 60 s. The Michaelis-Menten constant determined for this enzyme was 1.5 × 10-3 mol I-1. | en |
dc.format.extent | 397-399 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.title | Use of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determination | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | - |
dc.description.affiliation | Departamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP | - |
dc.description.affiliation | Instituto de Química Unicamp, PO Box 6154, 13083-970 Campinas, SP | - |
dc.description.affiliationUnesp | Departamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP | - |
dc.identifier.doi | 10.1039/AC9963300397 | - |
dc.identifier.wos | WOS:A1996VT08900003 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Analytical Communications | - |
dc.identifier.scopus | 2-s2.0-0000678174 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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