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http://acervodigital.unesp.br/handle/11449/65291
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DC Field | Value | Language |
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dc.contributor.author | Oliveira Neto, Graciliano de | - |
dc.contributor.author | Tubino, Matthieu | - |
dc.contributor.author | Godinho, Oswaldo Espirito Santo | - |
dc.contributor.author | Kubota, Lauro Tatsuo | - |
dc.contributor.author | Fernandes, João Roberto | - |
dc.date.accessioned | 2014-05-27T11:18:18Z | - |
dc.date.accessioned | 2016-10-25T18:14:47Z | - |
dc.date.available | 2014-05-27T11:18:18Z | - |
dc.date.available | 2016-10-25T18:14:47Z | - |
dc.date.issued | 1997-12-01 | - |
dc.identifier | http://dx.doi.org/10.1590/S0103-50531997000100009 | - |
dc.identifier.citation | Journal of the Brazilian Chemical Society, v. 8, n. 1, p. 47-51, 1997. | - |
dc.identifier.issn | 0103-5053 | - |
dc.identifier.uri | http://hdl.handle.net/11449/65291 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/65291 | - |
dc.description.abstract | A flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 μL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006(±0.016) + 0.98(±0.019)X; r = 0.9995, Y = conductivity in μS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about ± 3.2 % (r.s.d). | en |
dc.format.extent | 47-51 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | Conductimetry | - |
dc.subject | Enzyme immobilization | - |
dc.subject | Flow injection | - |
dc.subject | Oxalate determination | - |
dc.subject | Oxalate oxidase | - |
dc.subject | Sorghum vulgare | - |
dc.title | Oxalate determination in urine using an immobilized enzyme on Sorghum vulgare seeds in a flow injection conductimetric system | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ. Federal do Maranhão | - |
dc.description.affiliation | Inst. de Química da UNICAMP C.P. 6154, 13083-970 Campinas - SP | - |
dc.description.affiliation | Faculdade de Ciências Depto de Química UNESP, Av. Luis Edmundo C. Coube s/n, 17033-360 Bauru - SP | - |
dc.description.affiliation | Univ. Federal do Maranhão, São Luís | - |
dc.description.affiliationUnesp | Faculdade de Ciências Depto de Química UNESP, Av. Luis Edmundo C. Coube s/n, 17033-360 Bauru - SP | - |
dc.identifier.doi | 10.1590/S0103-50531997000100009 | - |
dc.identifier.scielo | S0103-50531997000100009 | - |
dc.identifier.wos | WOS:A1997WP60200009 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | 2-s2.0-3042869073.pdf | - |
dc.relation.ispartof | Journal of the Brazilian Chemical Society | - |
dc.identifier.scopus | 2-s2.0-3042869073 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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