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dc.contributor.authorJubilut, Guita N.-
dc.contributor.authorMarchetto, Reinaldo-
dc.contributor.authorCilli, Eduardo Maffud-
dc.contributor.authorOliveira, Eliandre-
dc.contributor.authorMiranda, Antonio-
dc.contributor.authorTominaga, Mineko-
dc.contributor.authorNakaie, Clóvis R.-
dc.identifier.citationJournal of the Brazilian Chemical Society, v. 8, n. 1, p. 65-70, 1997.-
dc.description.abstractThe classic hydrolysis procedure for quantification of resin-bound aminoacyl and peptidyl groups with 12 N HCl: propionic acid was recvaluated by studying the influence of the nature of the resin and the resin-bound group. Their stability during acid hydrolysis was dependent on the C-terminal amino acid, and the order of acid stability was Phe > Val > Gly. Otherwise, the dipeptides Ala-Gly, Ala-Val, and Ala-Phe displayed enhanced rates of hydrolysis of the resin if compared with their parent aminoacyl groups. Amongthe resins assayed, the order of acid stability was: benzhydrylamine-resin > p-methylbenzhydrylamine-resin ≅4-(oxymethyl)-phenylacetamidomethyl-resin > chloromethyl-copolymer of styrene-1%-divinylbenzene. Important for peptide synthesis method, the findings demonstrate that longer hydrolysis times than previously recommended in the literature (1 h at 130°C and 15 min at 160°C for peptides attached to the chloromethyl-copolymer of styrene-1%-divinylbenzene) are necessary for the quantitative acid-catalyzed cleavage of some resin-bound groups. The observed broad range of hydrolysis time varied from less than 1 h to about 100 h.en
dc.subjectAcid hydrolysis-
dc.subjectAcyl-resin hydrolysis-
dc.subjectAmino acid analysis-
dc.subjectPeptide hydrolysis-
dc.subjectSolid phase peptide synthesis-
dc.titleComparative time-course study of aminoacyl- and dipeptidyl-resin hydrolysisen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartamento de Biofisica Univ. Federal de São Paulo, Rua 3 de Maio 100, 04044-020 São Paulo - SP-
dc.description.affiliationDepartamento de Bioquímica Instituto de Química Universidade Estadual Paulista, 14800-060 Araraquara - SP-
dc.description.affiliationUnespDepartamento de Bioquímica Instituto de Química Universidade Estadual Paulista, 14800-060 Araraquara - SP-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofJournal of the Brazilian Chemical Society-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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