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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/65692
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dc.contributor.authorHeinemann, M. B.-
dc.contributor.authorGarcia, J. F.-
dc.contributor.authorNunes, C. M.-
dc.contributor.authorMorais, Z. M.-
dc.contributor.authorGregori, F.-
dc.contributor.authorCortez, A.-
dc.contributor.authorVasconcellos, S. A.-
dc.contributor.authorVisintin, J. A.-
dc.contributor.authorRichtzenhain, L. J.-
dc.date.accessioned2014-05-27T11:19:41Z-
dc.date.accessioned2016-10-25T18:15:33Z-
dc.date.available2014-05-27T11:19:41Z-
dc.date.available2016-10-25T18:15:33Z-
dc.date.issued1999-01-01-
dc.identifierhttp://dx.doi.org/10.1111/j.1751-0813.1999.tb12422.x-
dc.identifier.citationAustralian Veterinary Journal, v. 77, n. 1, p. 32-34, 1999.-
dc.identifier.issn0005-0423-
dc.identifier.urihttp://hdl.handle.net/11449/65692-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/65692-
dc.description.abstractObjective: In view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design: Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at artificial insemination centres in Brazil. Each animal was sampled twice for serology. Result: Forty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo in the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion: Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres.en
dc.format.extent32-34-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCattle-
dc.subjectLeptospirosis-
dc.subjectPCR-
dc.subjectSemen-
dc.subjectbacterium antibody-
dc.subjectanimal-
dc.subjectanimal disease-
dc.subjectartificial insemination-
dc.subjectblood-
dc.subjectBrazil-
dc.subjectcattle-
dc.subjectcattle disease-
dc.subjectdisease transmission-
dc.subjectfemale-
dc.subjectisolation and purification-
dc.subjectLeptospira-
dc.subjectleptospirosis-
dc.subjectmethodology-
dc.subjectmicrobiology-
dc.subjectpolymerase chain reaction-
dc.subjectspecies difference-
dc.subjectsperm-
dc.subjectAnimals-
dc.subjectAntibodies, Bacterial-
dc.subjectCattle Diseases-
dc.subjectFemale-
dc.subjectInsemination, Artificial-
dc.subjectPolymerase Chain Reaction-
dc.subjectSpecies Specificity-
dc.titleDetection of leptospires in bovine semen by polymerase chain reactionen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationSão Paulo State University UNESP Campus Araçatuba, R. Clovis Pestana, 793, Araçatuba, SP, 16050-680-
dc.description.affiliationFaculdade de Med. Vet. e Zootecnia University of São Paulo, Av. Prof. Dr. Orlando Marques Paiva, São Paulo, SP, 05508-000-
dc.description.affiliationUnespSão Paulo State University UNESP Campus Araçatuba, R. Clovis Pestana, 793, Araçatuba, SP, 16050-680-
dc.identifier.doi10.1111/j.1751-0813.1999.tb12422.x-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofAustralian Veterinary Journal-
dc.identifier.scopus2-s2.0-0032610351-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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