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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/66345
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dc.contributor.authorMartins, Cesar-
dc.contributor.authorWasko, Adriane P.-
dc.contributor.authorOliveira, Claudio-
dc.contributor.authorWright, Jonathan M.-
dc.date.accessioned2014-05-27T11:19:59Z-
dc.date.accessioned2016-10-25T18:16:43Z-
dc.date.available2014-05-27T11:19:59Z-
dc.date.available2016-10-25T18:16:43Z-
dc.date.issued2000-12-01-
dc.identifierhttp://dx.doi.org/10.1111/j.1601-5223.2000.00039.x-
dc.identifier.citationHereditas, v. 133, n. 1, p. 39-46, 2000.-
dc.identifier.issn0018-0661-
dc.identifier.urihttp://hdl.handle.net/11449/66345-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/66345-
dc.description.abstractIn this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci.en
dc.format.extent39-46-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectnucleotide-
dc.subjectribosome DNA-
dc.subjectRNA-
dc.subjectspacer DNA-
dc.subjectanimal genetics-
dc.subjectfemale-
dc.subjectgene deletion-
dc.subjectgene insertion-
dc.subjectgene locus-
dc.subjectgenetic analysis-
dc.subjectmale-
dc.subjectmetaphase chromosome-
dc.subjectnonhuman-
dc.subjectnucleotide sequence-
dc.subjectTilapia-
dc.subjectAnimals-
dc.subjectBase Sequence-
dc.subjectBlotting, Southern-
dc.subjectChromosome Mapping-
dc.subjectChromosomes-
dc.subjectDNA Primers-
dc.subjectDNA, Ribosomal-
dc.subjectElectrophoresis, Agar Gel-
dc.subjectIn Situ Hybridization, Fluorescence-
dc.subjectMetaphase-
dc.subjectMolecular Sequence Data-
dc.subjectPerches-
dc.subjectPolymerase Chain Reaction-
dc.subjectRNA, Ribosomal, 5S-
dc.subjectSequence Analysis, DNA-
dc.subjectSequence Homology, Nucleic Acid-
dc.subjectAnimalia-
dc.subjectCichlidae-
dc.subjectOreochromis niloticus-
dc.titleNucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticusen
dc.typeoutro-
dc.contributor.institutionDalhousie University-
dc.contributor.institutionUniversidade Federal de Slo Carlos-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Biology Dalhousie University, Halifax, NS B3H 4J1-
dc.description.affiliationDepartamento de Genktica e Evoluqlo Universidade Federal de Slo Carlos, Slo Carlos, SP-
dc.description.affiliationDepartamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SP-
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SP-
dc.identifier.doi10.1111/j.1601-5223.2000.00039.x-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-0034510558.pdf-
dc.relation.ispartofHereditas-
dc.identifier.scopus2-s2.0-0034510558-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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