You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/66849
Full metadata record
DC FieldValueLanguage
dc.contributor.authorFerreira, Lucia P-
dc.contributor.authorLemos, Eliana G.M-
dc.contributor.authorLemos, Manoel Victor F-
dc.date.accessioned2014-05-27T11:20:25Z-
dc.date.accessioned2016-10-25T18:17:42Z-
dc.date.available2014-05-27T11:20:25Z-
dc.date.available2016-10-25T18:17:42Z-
dc.date.issued2002-03-05-
dc.identifierhttp://dx.doi.org/10.1016/S0378-1097(01)00473-6-
dc.identifier.citationFEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.-
dc.identifier.issn0378-1097-
dc.identifier.urihttp://hdl.handle.net/11449/66849-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/66849-
dc.description.abstractTransposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.en
dc.format.extent163-168-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectPCR fragment-
dc.subjectTransposon Tn1721-
dc.subjectXylella fastidiosa-
dc.subjectbacterial DNA-
dc.subjectbacterial chromosome-
dc.subjectbacterial genome-
dc.subjectbacterial strain-
dc.subjectbacterium isolation-
dc.subjectcitrus fruit-
dc.subjectcontrolled study-
dc.subjectDNA template-
dc.subjectgene amplification-
dc.subjectgene deletion-
dc.subjectgene duplication-
dc.subjectgenetic variability-
dc.subjectGram negative bacterium-
dc.subjectnonhuman-
dc.subjectplum-
dc.subjectpolymerase chain reaction-
dc.subjectpriority journal-
dc.subjecttransposon-
dc.subjectxylell fastidiosa-
dc.subjectBase Sequence-
dc.subjectCitrus-
dc.subjectCoffee-
dc.subjectDNA Transposable Elements-
dc.subjectDNA, Bacterial-
dc.subjectGammaproteobacteria-
dc.subjectMolecular Sequence Data-
dc.subjectPlant Diseases-
dc.subjectPrunus-
dc.subjectSequence Alignment-
dc.subjectSpecies Specificity-
dc.subjectAnimalia-
dc.subjectBacteria (microorganisms)-
dc.subjectFungi-
dc.subjectPrunus domestica-
dc.subjecttransposons-
dc.subjectVitis-
dc.titleTransposon Tn1721 distribution among strains of Xylella fastidiosaen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 Jaboticabal-
dc.description.affiliationDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 Jaboticabal-
dc.description.affiliationUnespDepartmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 Jaboticabal-
dc.description.affiliationUnespDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 Jaboticabal-
dc.identifier.doi10.1016/S0378-1097(01)00473-6-
dc.identifier.wosWOS:000175152500002-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-0037022952.pdf-
dc.relation.ispartofFEMS Microbiology Letters-
dc.identifier.scopus2-s2.0-0037022952-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.