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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/67468
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dc.contributor.authorCaroci, A. S.-
dc.contributor.authorCalvo, E.-
dc.contributor.authorRibolla, P. E.-
dc.contributor.authorDe Bianchi, A. G.-
dc.contributor.authorMarinotti, O.-
dc.date.accessioned2014-05-27T11:20:56Z-
dc.date.accessioned2016-10-25T18:18:59Z-
dc.date.available2014-05-27T11:20:56Z-
dc.date.available2016-10-25T18:18:59Z-
dc.date.issued2003-11-01-
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/14765682-
dc.identifier.citationJournal of Medical Entomology, v. 40, n. 6, p. 991-995, 2003.-
dc.identifier.issn0022-2585-
dc.identifier.urihttp://hdl.handle.net/11449/67468-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/67468-
dc.description.abstractTrypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Amino-terminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.en
dc.format.extent991-995-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectAnopheles-
dc.subjectMalaria vector-
dc.subjectTrypsin-
dc.subjecttrypsin-
dc.subjectamino acid sequence-
dc.subjectanimal-
dc.subjectblood-
dc.subjectBrazil-
dc.subjectchemistry-
dc.subjectclassification-
dc.subjectcomparative study-
dc.subjectdigestion-
dc.subjectenzymology-
dc.subjectfeeding behavior-
dc.subjectgenetics-
dc.subjecthuman-
dc.subjectisolation and purification-
dc.subjectkinetics-
dc.subjectmetabolism-
dc.subjectmolecular genetics-
dc.subjectnucleotide sequence-
dc.subjectparasitology-
dc.subjectphysiology-
dc.subjectsensitivity and specificity-
dc.subjectsequence alignment-
dc.subjectsequence homology-
dc.subjectAmino Acid Sequence-
dc.subjectAnimals-
dc.subjectBlood-
dc.subjectConserved Sequence-
dc.subjectDigestion-
dc.subjectFeeding Behavior-
dc.subjectHumans-
dc.subjectKinetics-
dc.subjectMolecular Sequence Data-
dc.subjectSensitivity and Specificity-
dc.subjectSequence Alignment-
dc.subjectSequence Homology, Amino Acid-
dc.subjectAlbitarsis-
dc.subjectAnimalia-
dc.subjectAnopheles albitarsis-
dc.subjectAnopheles aquasalis-
dc.subjectAnopheles darlingi-
dc.subjectHexapoda-
dc.titleTwo Digestive Trypsins Occur in Three Species of Neotropical Anophelinesen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversity of California-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartamento de Parasitologia Inst. de Ciê. Bioméd. Universidade de São Paulo, São Paulo, SP, CEP 05508-900-
dc.description.affiliationDept. of Molec. Biol. and Biochem. University of California, Irvine, CA 92697-
dc.description.affiliationDepartamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP-
dc.description.affiliationUnespDepartamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Medical Entomology-
dc.identifier.scopus2-s2.0-1642529555-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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