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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/67819
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dc.contributor.authorVilas-Bôas, Gislayne Trindade-
dc.contributor.authorLemos, Manoel Victor Franco-
dc.date.accessioned2014-05-27T11:21:07Z-
dc.date.accessioned2016-10-25T18:19:46Z-
dc.date.available2014-05-27T11:21:07Z-
dc.date.available2016-10-25T18:19:46Z-
dc.date.issued2004-08-01-
dc.identifierhttp://dx.doi.org/10.1139/w04-052-
dc.identifier.citationCanadian Journal of Microbiology, v. 50, n. 8, p. 605-613, 2004.-
dc.identifier.issn0008-4166-
dc.identifier.urihttp://hdl.handle.net/11449/67819-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/67819-
dc.description.abstractTwo hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were selected according to their geographic origin for genetic characterization with the 16S rRNA gene, RAPD, and plasmid profile. Isolates containing cryl genes were the most abundant (48%) followed by the cry11 and cyt (7%) and cry8 genes (2%). Finally, 40.3% of the isolates did not produce any PCR product. The plasmid profile and RAPD analysis showed a remarkable diversity among the isolates of B. thuringiensis not observed in the 16S rRNA gene. These results suggest that the genetic diversity of B. thuringiensis species results from the influence of different ecological factors and spatial separation between strains generated by the conquest of different habitats.en
dc.format.extent605-613-
dc.language.isoeng-
dc.sourceScopus-
dc.subject16S rRNA gene-
dc.subjectBacillus thuringiensis-
dc.subjectCry genes-
dc.subjectEcology-
dc.subjectGenetic diversity-
dc.subjectPlasmid profile-
dc.subjectRAPD-
dc.subjectBiodiversity-
dc.subjectCrystal structure-
dc.subjectGenes-
dc.subjectProteins-
dc.subjectRNA-
dc.subjectGenetic characterization-
dc.subjectSpatial separation-
dc.subjectBacteria-
dc.subjectribosome RNA-
dc.subjectRNA 16S-
dc.subjectbacterium-
dc.subjectgenetic variation-
dc.subjectplasmid-
dc.subjectpolymerase chain reaction-
dc.subjectbacterial gene-
dc.subjectbacterium isolate-
dc.subjectBrazil-
dc.subjectcry gene-
dc.subjectcyt gene-
dc.subjectgenetic variability-
dc.subjectgeographic distribution-
dc.subjecthabitat structure-
dc.subjectnonhuman-
dc.subjectpriority journal-
dc.subjectrandom amplified polymorphic DNA-
dc.subjectRNA gene-
dc.subjectBacterial Proteins-
dc.subjectBacterial Toxins-
dc.subjectDNA, Ribosomal-
dc.subjectEndotoxins-
dc.subjectHemolysin Proteins-
dc.subjectMolecular Sequence Data-
dc.subjectPlasmids-
dc.subjectPolymerase Chain Reaction-
dc.subjectRandom Amplified Polymorphic DNA Technique-
dc.subjectRNA, Ribosomal, 16S-
dc.subjectSequence Analysis, DNA-
dc.subjectVariation (Genetics)-
dc.subjectSouth America-
dc.subjectWestern Hemisphere-
dc.subjectWorld-
dc.subjectBacteria (microorganisms)-
dc.subjectPosibacteria-
dc.titleDiversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazilen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepto. de Biol. Apl. a Agropecuaria FCAV/UNESP, Via Acesso Prof. Paulo D. Castellane, Jaboticabal/SP, 14884-900-
dc.description.affiliationDepartamento de Biologia Geral CCB UEL, CP 6001, Londrina/PR, 86051-990-
dc.description.affiliationUnespDepto. de Biol. Apl. a Agropecuaria FCAV/UNESP, Via Acesso Prof. Paulo D. Castellane, Jaboticabal/SP, 14884-900-
dc.identifier.doi10.1139/w04-052-
dc.identifier.wosWOS:000224734600007-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCanadian Journal of Microbiology-
dc.identifier.scopus2-s2.0-8644273112-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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