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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/68055
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dc.contributor.authorBarbieri, C. M O-
dc.contributor.authorCosta, Paulo Inácio da-
dc.date.accessioned2014-05-27T11:21:14Z-
dc.date.accessioned2016-10-25T18:20:18Z-
dc.date.available2014-05-27T11:21:14Z-
dc.date.available2016-10-25T18:20:18Z-
dc.date.issued2004-12-01-
dc.identifier.citationRevista de Ciencias Farmaceuticas, v. 25, n. 2, p. 129-134, 2004.-
dc.identifier.issn0101-3793-
dc.identifier.urihttp://hdl.handle.net/11449/68055-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/68055-
dc.description.abstractHuman platelet-derived growth factor (PDGF) was purified from lysates of clinically outdated human platelets by ionic exchange chromatography in CM-Sepharose. The eluated fraction was submitted to the Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB polyclonal antibodies and was evaluated as to its biological activity through the test of [H 3]-thymidine incorporation in NIH/3T3 cell line fibroblasts in culture. The Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB antibodies proved the presence of the PDGF in chromatographic cationic fraction. The comparison of biological activities between fiblobrast stimulation assay using recombinant PDGF-AB and partially purified PDGF was demonstrated in 165.796 and 157.567 cpm, respectively. This result, proved the potent mitogenic effect of partially purified PDGF and consequently their evidence about the wound healing activity.en
dc.format.extent129-134-
dc.language.isopor-
dc.sourceScopus-
dc.subjectPDGF-
dc.subjectPlatelets-
dc.subjectWound healing-
dc.subjectcation-
dc.subjectplatelet derived growth factor-
dc.subjectplatelet derived growth factor AA-
dc.subjectplatelet derived growth factor AB-
dc.subjectplatelet derived growth factor BB-
dc.subjectpolyclonal antibody-
dc.subjectrecombinant platelet derived growth factor-
dc.subjectsepharose-
dc.subjectthymidine-
dc.subjecttritium-
dc.subjectwound healing promoting agent-
dc.subjectanimal cell-
dc.subjectcell lysate-
dc.subjectcell stimulation-
dc.subjectcell strain 3T3-
dc.subjectcontrolled study-
dc.subjectdrug activity-
dc.subjectdrug cost-
dc.subjectdrug potency-
dc.subjectdrug purification-
dc.subjectdrug screening-
dc.subjectfibroblast culture-
dc.subjectimmunoblotting-
dc.subjection exchange chromatography-
dc.subjectmethodology-
dc.subjectmitogenesis-
dc.subjectmouse-
dc.subjectnonhuman-
dc.subjectslot blot hybridization-
dc.subjectthrombocyte-
dc.subjectwound healing-
dc.titleFator de crescimento derivado de plaquetas humanas obtido por uma metodologia rápida e de baixo custopt
dc.title.alternativeHuman platelet-derived growth factor obtained by means of a fast and inexpensive methodologyen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationHemonúcleo Regional de Araraquara Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP-
dc.description.affiliationDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP-
dc.description.affiliationUnespHemonúcleo Regional de Araraquara Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP-
dc.description.affiliationUnespDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, 14801-902 - Araraquara - SP-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofRevista de Ciencias Farmaceuticas-
dc.identifier.scopus2-s2.0-27444441116-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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