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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/68792
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Campo DCValorIdioma
dc.contributor.authorPenteado, Flora Cristina Lobo-
dc.contributor.authorMedeiros, Luciene-
dc.contributor.authorDelgado Orellana, Maristela-
dc.contributor.authorPalma, Patricia-
dc.contributor.authorFontes, Aparecida Maria-
dc.contributor.authorTakayanagui, Osvaldo Massaiti-
dc.contributor.authorCovas, Dimas Tadeu-
dc.date.accessioned2014-05-27T11:21:49Z-
dc.date.accessioned2016-10-25T18:21:58Z-
dc.date.available2014-05-27T11:21:49Z-
dc.date.available2016-10-25T18:21:58Z-
dc.date.issued2006-03-01-
dc.identifierhttp://dx.doi.org/10.1590/S0037-86822006000200007-
dc.identifier.citationRevista da Sociedade Brasileira de Medicina Tropical, v. 39, n. 2, p. 169-173, 2006.-
dc.identifier.issn0037-8682-
dc.identifier.urihttp://hdl.handle.net/11449/68792-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/68792-
dc.description.abstractThe retrovirus HTLV-1 is the etiological agent of the adult T-cell leukemia and HTLV-1 associated myelopathy/tropical spastic paraparesis. The proviral genome has 9,032 base pairs, showing regulatory and structural genes. The env gene encodes for the transmembrane glycoprotein gp 21. The development of methodologies for heterologous protein expression, as well as the acquisition of a cellular line that constituently expresses the recombinant, were the main goals of this work. The DNA fragment that encodes for gp 21 was amplified by nested-PCR and cloned into a pCR2.1-TOPO vector. After which, a sub-cloning was realized using the expressing vector pcDNA3.1+. The transfection of mammalian cells HEK 293 was performed transitorily and permanently. Production of the recombinant gp 21 was confirmed by flux cytometry experiments and the cell line producing protein will be used in immunogenicity assays.en
dc.format.extent169-173-
dc.language.isopor-
dc.sourceScopus-
dc.subjectCloning-
dc.subjectHTLV-1-
dc.subjectProtein expression-
dc.subjectTransmembrane glycoprotein-
dc.subjectDNA fragment-
dc.subjectglycoprotein-
dc.subjectmembrane protein-
dc.subjectrecombinant protein-
dc.subjectanimal cell-
dc.subjectcell line-
dc.subjectcytometry-
dc.subjectenvelope gene-
dc.subjectgene control-
dc.subjectgenetic transfection-
dc.subjectHuman T cell leukemia virus 1-
dc.subjectimmunogenicity-
dc.subjectmolecular cloning-
dc.subjectnonhuman-
dc.subjectpolymerase chain reaction-
dc.subjectprotein blood level-
dc.subjectprotein expression-
dc.subjectRetrovirus-
dc.subjectspastic paresis-
dc.subjectspinal cord disease-
dc.subjectstructural gene-
dc.subjectT cell leukemia-
dc.subjecttropical disease-
dc.subjectvirus gene-
dc.subjectAnimals-
dc.subjectCell Lineage-
dc.subjectCloning, Organism-
dc.subjectFlow Cytometry-
dc.subjectGene Expression Regulation, Developmental-
dc.subjectHuman T-lymphotropic virus 1-
dc.subjectMammals-
dc.subjectMembrane Glycoproteins-
dc.subjectPolymerase Chain Reaction-
dc.subjectViral Envelope Proteins-
dc.subjectAnimalia-
dc.subjectMammalia-
dc.titleClonagem e expressão da glicoproteína transmembrana do retrovírus HTLV-1 em células de mamíferospt
dc.title.alternativeCloning and transmembrane glycoprotein expression of the retrovirus HTLV-1 in mammals' cellsen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationLaboratório de Pesquisa Centro Regional de Hemoterapia Universidade de São Paulo, Ribeirão Preto, SP-
dc.description.affiliationDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SP-
dc.description.affiliationDepartamento de Neurologia Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SP-
dc.description.affiliationDepartamento de Clinica Medica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SP-
dc.description.affiliationCentro Regional de Hemoterapia HCFMRP/Hemocentro de Ribeirão Preto. R. Tenente, Catão Roxo 2501, 14051-140 Ribeirão Preto, SP-
dc.description.affiliationUnespDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SP-
dc.identifier.doi10.1590/S0037-86822006000200007-
dc.identifier.scieloS0037-86822006000200007-
dc.identifier.wosWOS:000237180400007-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-33646676282.pdf-
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical-
dc.identifier.scopus2-s2.0-33646676282-
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