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- Título:
- Monomolecular films of cholesterol oxidase and S-Layer proteins
- Universidade Federal do Rio de Janeiro (UFRJ)
- Universidade Estadual Paulista (UNESP)
- 0169-4332
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- CNPq: 62/2008
- Cholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (pi-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. (C) 2011 Elsevier B. V. All rights reserved.
- 15-Mai-2011
- Applied Surface Science. Amsterdam: Elsevier B.V., v. 257, n. 15, p. 6535-6539, 2011.
- 6535-6539
- Elsevier B.V.
- Cholesterol
- Cholesterol oxidase
- S-Layer proteins
- Monolayer
- Biosensor
- http://dx.doi.org/10.1016/j.apsusc.2011.01.143
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/6881
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