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dc.contributor.authorFerraz, Helen Conceicao-
dc.contributor.authorGuimaraes, Juliana Aguilar-
dc.contributor.authorMoitinho Alves, Tito Livio-
dc.contributor.authorLeopoldo Constantino, Carlos Jose-
dc.identifier.citationApplied Surface Science. Amsterdam: Elsevier B.V., v. 257, n. 15, p. 6535-6539, 2011.-
dc.description.abstractCholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (pi-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. (C) 2011 Elsevier B. V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectCholesterol oxidaseen
dc.subjectS-Layer proteinsen
dc.titleMonomolecular films of cholesterol oxidase and S-Layer proteinsen
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Fed Rio de Janeiro, Chem Engn Program, BR-21941972 Rio de Janeiro, Brazil-
dc.description.affiliationUniv Estadual Paulista, Dept Phys Chem & Biol, BR-19060900 Presidente Prudente, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Phys Chem & Biol, BR-19060900 Presidente Prudente, SP, Brazil-
dc.description.sponsorshipIdCNPq: 62/2008-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofApplied Surface Science-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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