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- Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated (1 -> 3)(1 -> 6)-beta-D-Glucan
- Universidade Estadual Paulista (UNESP)
- Lakehead Univ
- Universidade Estadual de Londrina (UEL)
- Universidade Federal do Paraná (UFPR)
- Universidade Federal do Rio de Janeiro (UFRJ)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)
- Programa de Apoio aos Núcleos de Excelência (PRONEX)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- FAPESP: 05/53879-3
- FAPESP: 05/50936-6
- Fundação Araucaria: 5777
- The exopolysaccharide botryosphaeran (EPS(GLC); a (1 -> 3)(1 -> 6)-beta-D-glucan from Botryosphaeria rhodina MAMB-05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPS(GLC)-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and (13)C nuclear magnetic resonance ((13)C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant.
- Journal of Microbiology and Biotechnology. Seoul: Korean Soc Microbiology & Biotechnology, v. 21, n. 10, p. 1036-1042, 2011.
- Korean Soc Microbiology & Biotechnology
- anticoagulant activity
- Botryosphaeria rhodina MAMB-05
- Acesso restrito
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