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dc.contributor.authorApetrei, C.-
dc.contributor.authorAlessio, P.-
dc.contributor.authorConstantino, C. J. L.-
dc.contributor.authorde Saja, J. A.-
dc.contributor.authorRodriguez-Mendez, M. L.-
dc.contributor.authorPavinatto, F. J.-
dc.contributor.authorRamos Fernandes, E. Giuliani-
dc.contributor.authorZucolotto, V.-
dc.contributor.authorOliveira, O. N.-
dc.identifier.citationBiosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, v. 26, n. 5, p. 2513-2519, 2011.-
dc.description.abstractThis paper describes the preparation of a biomimetic Langmuir-Blodgett film of tyrosinase incorporated in a lipidic layer and the use of lutetium bisphthalocyanine as an electron mediator for the voltammetric detection of phenol derivatives, which include one monophenol (vanillic acid), two diphenols (catechol and caffeic acid) and two triphenols (gallic acid and pyrogallol). The first redox process of the voltammetric responses is associated with the reduction of the enzymatically formed o-quinone and is favoured by the lutetium bisphthalocyanine because significant signal amplification is observed, while the second is associated with the electrochemical oxidation of the antioxidant and occurs at lower potentials in the presence of an electron mediator. The biosensor shows low detection limit (1.98 x 10(-6)-27.49 x 10(-6) M), good reproducibility, and high affinity to antioxidants (Km in the range of 62.31-144.87 mu M). The excellent functionality of the enzyme obtained using a biomimetic immobilisation method, the selectivity afforded by enzyme catalysis, the signal enhancement caused by the lutetium bisphthalocyanine mediator and the increased selectivity of the curves due to the occurrence of two redox processes make these sensors exceptionally suitable for the detection of phenolic compounds. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipSpanish Ministry of Science-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherElsevier Advanced Technology-
dc.sourceWeb of Science-
dc.titleBiomimetic biosensor based on lipidic layers containing tyrosinase and lutetium bisphthalocyanine for the detection of antioxidantsen
dc.contributor.institutionUniv Valladolid-
dc.contributor.institutionDunarea de Jos Univ Galati-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationUniv Valladolid, Dept Inorgan Chem, ETS Ingn Ind, E-47011 Valladolid, Spain-
dc.description.affiliationDunarea de Jos Univ Galati, Fac Sci, Dept Chem, Galati 800008, Romania-
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil-
dc.description.affiliationUniv Valladolid, Fac Sci, Dept Condensed Matter Phys, E-47011 Valladolid, Spain-
dc.description.affiliationUniv São Paulo, Inst Fis São Carlos, BR-13560970 São Carlos, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil-
dc.description.sponsorshipIdSpanish Ministry of Science: AGL2009-12660/ALI-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiosensors & Bioelectronics-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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