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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/69276
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dc.contributor.authorDi Mauro, Antonio Orlando-
dc.contributor.authorDi Mauro, Daniela Cristina-
dc.contributor.authorCapeloto, Andrea-
dc.contributor.authorArriel, Nair Helena de Castro-
dc.contributor.authorCosta, Marcelo Marchi-
dc.contributor.authorTrevisoli, Sandra Helena Uneda-
dc.contributor.authorDi Mauro, Sonia Marli Zingaretti-
dc.date.accessioned2014-05-27T11:22:03Z-
dc.date.accessioned2016-10-25T18:23:04Z-
dc.date.available2014-05-27T11:22:03Z-
dc.date.available2016-10-25T18:23:04Z-
dc.date.issued2006-12-01-
dc.identifierhttp://dx.doi.org/10.12702/1984-7033.v06n02a08-
dc.identifier.citationCrop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006.-
dc.identifier.issn1518-7853-
dc.identifier.urihttp://hdl.handle.net/11449/69276-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/69276-
dc.description.abstractThe Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established.en
dc.format.extent167-170-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectConditions, watermelon, sesame-
dc.subjectDNA amplification-
dc.subjectCitrullus lanatus var. lanatus-
dc.subjectSesamum indicum-
dc.titleRAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypesen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)-
dc.description.affiliationDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP-
dc.description.affiliationDepartamento de Tecnologia UNESP-
dc.description.affiliationEmbrapa Algodão, Rua Oswaldo Cruz, 1143, Centenário, 58.107-720, Campina Grande, PB-
dc.description.affiliationAPTA Regional Centro Leste, C. P. 271, 14.001-970, Ribeirão Preto, SP-
dc.description.affiliationUnespDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP-
dc.description.affiliationUnespDepartamento de Tecnologia UNESP-
dc.identifier.doi10.12702/1984-7033.v06n02a08-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-41149127363.pdf-
dc.relation.ispartofCrop Breeding and Applied Biotechnology-
dc.identifier.scopus2-s2.0-41149127363-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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