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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/69538
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dc.contributor.authorCampanha, Nara Hellen-
dc.contributor.authorPavarina, Ana Claudia-
dc.contributor.authorBrunetti, Iguatemy Lourenço-
dc.contributor.authorVergani, Carlos Eduardo-
dc.contributor.authorMachado, Ana Lucia-
dc.contributor.authorSpolidório, Denise Madalena Palomari-
dc.date.accessioned2014-05-27T11:22:25Z-
dc.date.accessioned2016-10-25T18:23:36Z-
dc.date.available2014-05-27T11:22:25Z-
dc.date.available2016-10-25T18:23:36Z-
dc.date.issued2007-03-01-
dc.identifierhttp://dx.doi.org/10.1111/j.1439-0507.2006.01339.x-
dc.identifier.citationMycoses, v. 50, n. 2, p. 140-147, 2007.-
dc.identifier.issn0933-7407-
dc.identifier.issn1439-0507-
dc.identifier.urihttp://hdl.handle.net/11449/69538-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/69538-
dc.description.abstractIn indicating the microwave irradiation for disinfecting dentures it is necessary to see how this procedure influences Candida albicans integrity and viability. The aim of this study was to evaluate the ability of microwaves to inactivate C. albicans and damage cell membrane integrity. Two 200-ml C. albicans (ATCC 10231) suspensions were obtained. A sterile denture was placed in a beaker containing the Experimental (ES) or the Control suspension (CS). ES was microwaved at 650 W for 6 min. Suspensions were optically counted using methylene blue dye uptake as indicative of membrane-damaged cells; spread on Agar Sabouraud dextrose (ASD) for viability assay; or spectrophotometrically measured at 550 nm. Cell-free solutions were submitted to content analyses of protein (Bradford and Pyrogallol red methods); Ca++ (Cresolftaleine complexone method); DNA (spectrophotometer measurements at 260 nm) and K + (selective electrode technique). Data were analysed by Student's t- or Wilcoxon z-tests (α = 0.05). All ES cells demonstrated cell membrane damage. Viable cells were non-existent in the ES ASD plates. No significant difference in optical density between ES and CS was observed (P = 0.272). ES cells released significantly high protein (P < 0.001, Bradford; P = 0.005, Pyrogallol red), K+ (P < 0.001), Ca++ (P = 0.012) and DNA (P = 0.046) contents. Microwaves inactivated C. albicans and damaged cell membrane integrity. © 2007 The Authors.en
dc.format.extent140-147-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCandida albicans-
dc.subjectComplete-
dc.subjectDenture-
dc.subjectDenture stomatitis-
dc.subjectDisinfection-
dc.subjectMicrowaves-
dc.subjectYeast-
dc.subjectcalcium-
dc.subjectDNA-
dc.subjectmethylene blue-
dc.subjectpotassium ion-
dc.subjectagar medium-
dc.subjectcell free system-
dc.subjectcell membrane-
dc.subjectcell viability-
dc.subjectcontent analysis-
dc.subjectcontrolled study-
dc.subjectdenture-
dc.subjectDNA content-
dc.subjectDNA determination-
dc.subjectelectrode-
dc.subjectmembrane damage-
dc.subjectmicrowave irradiation-
dc.subjectnonhuman-
dc.subjectoptical density-
dc.subjectpriority journal-
dc.subjectprotein analysis-
dc.subjectrank sum test-
dc.subjectspectrophotometer-
dc.subjectspectrophotometry-
dc.subjectStudent t test-
dc.subjectsuspension-
dc.subjectCalcium-
dc.subjectCell Membrane-
dc.subjectColony Count, Microbial-
dc.subjectDNA, Fungal-
dc.subjectFungal Proteins-
dc.subjectMethylene Blue-
dc.subjectMicrobial Viability-
dc.subjectPermeability-
dc.subjectPotassium-
dc.subjectSpectrophotometry-
dc.titleCandida albicans inactivation and cell membrane integrity damage by microwave irradiationen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual de Ponta Grossa (UEPG)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Dentistry Ponta Grossa State University, Paraná-
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Araraquara Dental School São Paulo State University-UNESP, São Paulo-
dc.description.affiliationDepartment of Clinical Analyses Araraquara School of Pharmacy São Paulo State University-UNESP, São Paulo-
dc.description.affiliationDepartment of Dental Materials and Prosthodontics-UNESP Araraquara Dental School São Paulo State University, São Paulo-
dc.description.affiliationDepartment of Physiology and Pathology Araraquara Dental School São Paulo State University-UNESP, São Paulo-
dc.description.affiliationFaculdade de Odontologia de Araraquara - UNESP, Rua Humaitá, No. 1680, Araraquara - SP, C.E.P.: 14801-903-
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Araraquara Dental School São Paulo State University-UNESP, São Paulo-
dc.description.affiliationUnespDepartment of Clinical Analyses Araraquara School of Pharmacy São Paulo State University-UNESP, São Paulo-
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics-UNESP Araraquara Dental School São Paulo State University, São Paulo-
dc.description.affiliationUnespDepartment of Physiology and Pathology Araraquara Dental School São Paulo State University-UNESP, São Paulo-
dc.description.affiliationUnespFaculdade de Odontologia de Araraquara - UNESP, Rua Humaitá, No. 1680, Araraquara - SP, C.E.P.: 14801-903-
dc.identifier.doi10.1111/j.1439-0507.2006.01339.x-
dc.identifier.wosWOS:000244245900009-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofMycoses-
dc.identifier.scopus2-s2.0-33846949716-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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