Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/69542
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Julia, M. Julia B.F. | - |
dc.contributor.author | Borges, Alexandre Secorun | - |
dc.contributor.author | Nydam, Daryl V. | - |
dc.contributor.author | Horohov, David W. | - |
dc.contributor.author | Hecker, Rolf | - |
dc.contributor.author | Matychak, Mary Beth | - |
dc.date.accessioned | 2014-05-27T11:22:25Z | - |
dc.date.accessioned | 2016-10-25T18:23:37Z | - |
dc.date.available | 2014-05-27T11:22:25Z | - |
dc.date.available | 2016-10-25T18:23:37Z | - |
dc.date.issued | 2007-03-01 | - |
dc.identifier | http://dx.doi.org/10.1186/1476-8518-5-1 | - |
dc.identifier.citation | Journal of Immune Based Therapies and Vaccines, v. 5. | - |
dc.identifier.issn | 1476-8518 | - |
dc.identifier.uri | http://hdl.handle.net/11449/69542 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/69542 | - |
dc.description.abstract | Background: Cytosine-phosphate-guanosine oligodeoxynucleotide (CpG-ODN) has been used successfully to induce immune responses against viral and intracellular organisms in mammals. The main objective of this study was to test the effect of CpG-ODN on antigen presenting cells of young foals. Methods: Peripheral blood monocytes of foals (n = 7) were isolated in the first day of life and monthly thereafter up to 3 months of life. Adult horse (n = 7) monocytes were isolated and tested once for comparison. Isolated monocytes were stimulated with IL-4 and GM-CSF (to obtain dendritic cells, DC) or not stimulated (to obtain macrophages). Macrophages and DCs were stimulated for 14-16 hours with either CpG-ODN, LPS or not stimulated. The stimulated and non-stimulated cells were tested for cell surface markers (CD86 and MHC class II) using flow cytometry, mRNA expression of cytokines (IL-12, IFNα, IL-10) and TLR-9 using real time quantitative RT-PCR, and for the activation of the transcription factor NF-κB p65 using a chemiluminescence assay. Results: The median fluorescence of the MHC class II molecule in non-stimulated foal macrophages and DCs at birth were 12.5 times and 11.2 times inferior, respectively, than adult horse cells (p = 0.009). That difference subsided at 3 months of life (p = 0.3). The expression of the CD86 co-stimulatory molecule was comparable in adult horse and foal macrophages and DCs, independent of treatment. CpG-ODN stimulation induced IL-12p40 (53 times) and IFNα (23 times) mRNA expression in CpG-ODN-treated adult horse DCs (p = 0.078), but not macrophages, in comparison to non-stimulated cells. In contrast, foal APCs did not respond to CpG-ODN stimulation with increased cytokine mRNA expression up to 3 months of age. TLR-9 mRNA expression and NF-kB activation (NF-kB p65) in foal DCs and macrophages were comparable (p > 0.05) to adult horse cells. Conclusion: CpG-ODN treatment did not induce specific maturation and cytokine expression in foal macrophages and DCs. Nevertheless, adult horse DCs, but not macrophages, increased their expression of IL-12 and IFNα cytokines upon CpG-ODN stimulation. Importantly, foals presented an age-dependent limitation in the expression of MHC class II in macrophages and DCs, independent of treatment. © 2007 Flaminio et al; licensee BioMed Central Ltd. | en |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | alpha interferon | - |
dc.subject | CD86 antigen | - |
dc.subject | cell surface marker | - |
dc.subject | CpG oligodeoxynucleotide | - |
dc.subject | cytokine | - |
dc.subject | granulocyte macrophage colony stimulating factor | - |
dc.subject | immunoglobulin enhancer binding protein | - |
dc.subject | interleukin 10 | - |
dc.subject | interleukin 12 | - |
dc.subject | interleukin 4 | - |
dc.subject | lipopolysaccharide | - |
dc.subject | major histocompatibility antigen class 2 | - |
dc.subject | messenger RNA | - |
dc.subject | toll like receptor 9 | - |
dc.subject | animal cell | - |
dc.subject | antigen presenting cell | - |
dc.subject | cell isolation | - |
dc.subject | cell stimulation | - |
dc.subject | chemoluminescence | - |
dc.subject | controlled study | - |
dc.subject | dendritic cell | - |
dc.subject | flow cytometry | - |
dc.subject | foal | - |
dc.subject | gene expression | - |
dc.subject | horse | - |
dc.subject | macrophage | - |
dc.subject | monocyte | - |
dc.subject | nonhuman | - |
dc.subject | reverse transcription polymerase chain reaction | - |
dc.title | The effect of CpG-ODN on antigen presenting cells of the foal | en |
dc.type | outro | - |
dc.contributor.institution | Cornell University | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | University of Kentucky | - |
dc.contributor.institution | Qiagen GmbH | - |
dc.description.affiliation | Department of Clinical Sciences College of Veterinary Medicine Cornell University, Ithaca, NY | - |
dc.description.affiliation | Departamento de Clinica Veterinaria Faculdade de Medicina Veterinaria e Zootecnia Universidade Estadual Paulista 'Julio de Mesquita Filho', UNESP-Campus de Botucatu, Botucatu, SP | - |
dc.description.affiliation | Department of Population Medicine and Diagnostics Sciences College of Veterinary Medicine Cornell University, Ithaca, NY | - |
dc.description.affiliation | Department of Veterinary Science Maxwell H. Gluck Equine Research Center University of Kentucky, Lexington, KY | - |
dc.description.affiliation | Qiagen GmbH, Hilden | - |
dc.description.affiliationUnesp | Departamento de Clinica Veterinaria Faculdade de Medicina Veterinaria e Zootecnia Universidade Estadual Paulista 'Julio de Mesquita Filho', UNESP-Campus de Botucatu, Botucatu, SP | - |
dc.identifier.doi | 10.1186/1476-8518-5-1 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | 2-s2.0-33847190253.pdf | - |
dc.relation.ispartof | Journal of Immune Based Therapies and Vaccines | - |
dc.identifier.scopus | 2-s2.0-33847190253 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.