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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/69876
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dc.contributor.authorBonini-Domingos, C. R.-
dc.contributor.authorSilva, M. B.-
dc.contributor.authorRomero, R. M.-
dc.contributor.authorZamaro, P. J A-
dc.contributor.authorOndei, L. S.-
dc.contributor.authorZago, C. E S-
dc.contributor.authorMoreira, S. B.-
dc.contributor.authorSalgado, C. G.-
dc.date.accessioned2014-05-27T11:22:35Z-
dc.date.accessioned2016-10-25T18:24:18Z-
dc.date.available2014-05-27T11:22:35Z-
dc.date.available2016-10-25T18:24:18Z-
dc.date.issued2007-09-03-
dc.identifierhttp://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0301.pdf-
dc.identifier.citationGenetics and Molecular Research, v. 6, n. 2, p. 415-421, 2007.-
dc.identifier.issn1676-5680-
dc.identifier.urihttp://hdl.handle.net/11449/69876-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/69876-
dc.description.abstractStudies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures at different pH, isoelectric focusing, globin chain electrophoresis, and HPLC. The hematocrit (31 ± 2%) and total hemoglobin (7.5 ± 0.2 g/dL) values did not indicate gender variations. Alkaline pH electrophoresis of the total blood samples treated with 1% saponin demonstrated the presence of four well-defined hemoglobin fractions, one major component (fraction I), showing cathodic migration and three others faster than fraction I with anodic migration. When the samples were precipitated with chloroform, only two hemoglobin fractions were observed, similar to fractions I and III from the first procedure. Isoelectric focusing and HPLC showed the same pattern. With acid and neutral pH electrophoresis, two fractions with anodic migration were observed. The globin chain identification at alkaline pH showed two fractions, but four fractions were observed at acidic pH, suggesting that different polypeptide chains are involved in the hemoglobin molecule. The chromatographic separation of the total blood sample demonstrated that the major fraction comprised 81.9% and the minor 18.1%. The results obtained demonstrated a similarity between these hemoglobin components and those of some Chelidae reported in the literature for both land and aquatic animals, reflecting the adaptation to environmental conditions. ©FUNPEC-RP.en
dc.format.extent415-421-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectElectrophoretic profile-
dc.subjectHemoglobin polymorphism-
dc.subjectHemoglobins-
dc.subjectRhinoclemmys punctularia-
dc.subjectchloroform-
dc.subjecthemoglobin-
dc.subjectalkalinity-
dc.subjectcontrolled study-
dc.subjectelectrophoresis-
dc.subjectfemale-
dc.subjecthematocrit-
dc.subjecthemoglobin determination-
dc.subjecthigh performance liquid chromatography-
dc.subjectisoelectric focusing-
dc.subjectmale-
dc.subjectnonhuman-
dc.subjectpH-
dc.subjectpH measurement-
dc.subjectsex difference-
dc.subjectturtle-
dc.subjectAnimals-
dc.subjectChromatography-
dc.subjectEcology-
dc.subjectElectrophoresis-
dc.subjectFemale-
dc.subjectHematocrit-
dc.subjectHumans-
dc.subjectMale-
dc.subjectModels, Genetic-
dc.subjectPhylogeny-
dc.subjectPolymorphism, Genetic-
dc.subjectReptiles-
dc.subjectTurtles-
dc.subjectAnimalia-
dc.subjectChelidae-
dc.subjectReptilia-
dc.titleDescription of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctulariaen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionMuseu Paraense Emilio Goeldi-
dc.contributor.institutionUniversidade Federal do Pará (UFPA)-
dc.description.affiliationLaboratório de Hemoglobinas e Genética Das Doenças Hematológicas Departamento de Biologia IBILCE, S. José do Rio Preto, SP-
dc.description.affiliationMuseu Paraense Emilio Goeldi, Belém, PA-
dc.description.affiliationLaboratório de Dermatoimunologia UEPA/UFPA/MC, Belém, PA-
dc.description.affiliationUnespLaboratório de Hemoglobinas e Genética Das Doenças Hematológicas Departamento de Biologia IBILCE, S. José do Rio Preto, SP-
dc.identifier.wosWOS:000251696400019-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-34548282227.pdf-
dc.relation.ispartofGenetics and Molecular Research-
dc.identifier.scopus2-s2.0-34548282227-
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