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dc.contributor.authorSantos, I. Walter-
dc.contributor.authorBinsfeld, L. C.-
dc.contributor.authorWeiss, R. R.-
dc.contributor.authorKozicki, L. E.-
dc.contributor.authorRochadelli, R.-
dc.contributor.authorResende, M. V.-
dc.contributor.authorHossepian de Lima, V. F M-
dc.identifier.citationArchives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007.-
dc.description.abstractThe objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm.en
dc.subjectCanis familiaris-
dc.titleCryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial inseminationen
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionPontifícia Universidade Católica do Paraná (PUCPR)-
dc.description.affiliationDepartamento de Medicina Veterinária UFPR, Rua Pioneiro 2153, 85950-000 Palotina, PR-
dc.description.affiliationDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SP-
dc.description.affiliationDepartamento de Medicina Veterinária UFPR, Curitiba, SP-
dc.description.affiliationDepartamento de Medicina Veterinária PUCPR, Sao Jose dos Pinhais, PR-
dc.description.affiliationUnespDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SP-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofArchives of Veterinary Science-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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