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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/70356
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dc.contributor.authorJustulin Jr., Luis A.-
dc.contributor.authorDelella, Flavia K.-
dc.contributor.authorFelisbino, Sérgio L.-
dc.date.accessioned2014-05-27T11:22:50Z-
dc.date.accessioned2016-10-25T18:25:22Z-
dc.date.available2014-05-27T11:22:50Z-
dc.date.available2016-10-25T18:25:22Z-
dc.date.issued2008-04-01-
dc.identifierhttp://dx.doi.org/10.1007/s00441-007-0559-3-
dc.identifier.citationCell and Tissue Research, v. 332, n. 1, p. 171-183, 2008.-
dc.identifier.issn0302-766X-
dc.identifier.urihttp://hdl.handle.net/11449/70356-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/70356-
dc.description.abstractWe investigated the effects of doxazosin (Dox), an alpha-adrenoceptor antagonist used clinically for the treatment of benign prostatic hyperplasia (BPH), on the rat prostatic complex by assessing structural parameters, collagen fiber content, cell proliferation, and apoptosis. Adult Wistar rats were treated with Dox (25 mg/kg per day), and the ventral (VP), dorsolateral, and anterior prostate (AP) regions of the prostate complex were excised at 3, 7, and 30 days after treatment. At 24 h before being killed, the rats were injected once with 5-bromodeoxyuridine (BrdU; thymidine analog) to label mitotically active cells. The prostates were weighed and processed for histochemistry, morphometry-stereology, immunohistochemistry for BrdU, Western blotting for proliferating cell nuclear antigen (PCNA), and the TUNEL reaction for apoptosis. Dox-treated prostate lobes at day 3 presented increased weight, an enlarged ductal lumen, low cubical epithelial cells, reduced epithelial folds, and stretched smooth muscle cells. However, at day 30, the prostates exhibited a weight reduction of ∼20% and an increased area of collagen and reticular fibers in the stromal space. Dox also reduced epithelial cell proliferation and increased apoptosis in the three prostatic lobes. Western blotting for PCNA confirmed the reduction of cell proliferation by Dox, with the AP and VP being more affected than the dorsal prostate. Thus, Dox treatment alters epithelial cell behavior and prostatic tissue mechanical demand, inducing tissue remodeling in which collagen fibers assume a major role. © 2007 Springer-Verlag.en
dc.format.extent171-183-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectApoptosis-
dc.subjectCell proliferation-
dc.subjectCollagen-
dc.subjectDoxazosin-
dc.subjectProstate-
dc.subjectRat (Wistar, adult)-
dc.subjectSmooth muscle cell-
dc.subjectcollagen fiber-
dc.subjectcycline-
dc.subjectdoxazosin mesylate-
dc.subjectanimal experiment-
dc.subjectanimal tissue-
dc.subjectapoptosis-
dc.subjectcell proliferation-
dc.subjectcontrolled study-
dc.subjecthistochemistry-
dc.subjectimmunohistochemistry-
dc.subjectmale-
dc.subjectmorphometrics-
dc.subjectnick end labeling-
dc.subjectnonhuman-
dc.subjectpriority journal-
dc.subjectprostate-
dc.subjectprostate epithelium-
dc.subjectprostate hypertrophy-
dc.subjectprotein determination-
dc.subjectrat-
dc.subjectsmooth muscle fiber-
dc.subjectstereology-
dc.subjectWestern blotting-
dc.subjectWistar rat-
dc.subjectAnimals-
dc.subjectBody Weight-
dc.subjectCell Proliferation-
dc.subjectEpithelial Cells-
dc.subjectIn Situ Nick-End Labeling-
dc.subjectMale-
dc.subjectOrgan Size-
dc.subjectProliferating Cell Nuclear Antigen-
dc.subjectRats-
dc.subjectRats, Wistar-
dc.subjectRattus-
dc.subjectRattus norvegicus-
dc.titleDoxazosin reduces cell proliferation and increases collagen fibers in rat prostatic lobesen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Cell Biology Institute of Biology State University of Campinas (UNICAMP), Campinas, SP-
dc.description.affiliationDepartment of Morphology Institute of Biosciences São Paulo State University, 18.618-000 Botucatu, SP-
dc.description.affiliationUnespDepartment of Morphology Institute of Biosciences São Paulo State University, 18.618-000 Botucatu, SP-
dc.identifier.doi10.1007/s00441-007-0559-3-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCell and Tissue Research-
dc.identifier.scopus2-s2.0-41049086678-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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