You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGarcia, João Luis-
dc.contributor.authorGennari, Solange Maria-
dc.contributor.authorNavarro, Italmar Teodorico-
dc.contributor.authorMachado, Rosângela Zacarias-
dc.contributor.authorHeadley, Selwyn Arligton-
dc.contributor.authorVidotto, Odilon-
dc.contributor.authorda Silva Guimarães Jr., José-
dc.contributor.authorBugni, Felipe Monteiro-
dc.contributor.authorIgarashi, Michelle-
dc.identifier.citationResearch in Veterinary Science, v. 84, n. 2, p. 237-242, 2008.-
dc.description.abstractThe study evaluated the efficiency of diagnostic laboratory methods to detect anti-Toxoplasma gondii antibodies in paired serum and aqueous humour samples from experimentally infected pigs. 18-mixed breed pigs were used during the experiment; these were divided into two groups, G1 (infected group, n = 10) and G2 (uninfected group, n = 8). Infection was performed with 4 × 10 4 VEG strain oocysts at day 0 by the oral route in G1 animals. All pigs were euthanized at day 60, when retina, aqueous humour, and blood samples were collected. Anti-T. gondii antibody levels were assessed in serum (s) and aqueous humour (ah) by indirect immunofluorescence assay (IFA), modified agglutination test (MAT), m-ELISA (using crude membranes from T. gondii tachyzoites as antigen) and r-ELISA (using rhoptries from T. gondii tachyzoites as antigen). Polymerase chain reactions (PCR) of samples from the retina were performed by using Tox4 and Tox5 primers. Antibody titers of G1 animals ranged from 128 to 1024 and from 16 to 256 in serum and aqueous humour, respectively. There were differences in the correlation coefficients between IFA(s) × IFA (ah) (r = 0.62, P = 0.05), MAT(s) × MAT (ah) (r = 0.97, P < 0.0001); however, there was no significant difference between r-ELISA(s) × r-ELISA (ah) (r = 0.14, P = 0.7). Antibodies present in serum and aqueous humour recognized similar antigens. Samples of retina were positive by PCR in 30% (3/10) of infected pigs. G2 animals remained without antibody levels and were PCR negative throughout the experiment. These results suggest that the use of a combination of tests and immunoblotting for paired aqueous humour and serum samples could improve the sensitivity and specificity for the diagnosis of ocular toxoplasmosis. © 2007 Elsevier Ltd. All rights reserved.en
dc.subjectAqueous humour-
dc.subjectToxoplasma gondii-
dc.subjectprotozoon antibody-
dc.subjectagglutination test-
dc.subjectanimal euthanasia-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectanimal tissue-
dc.subjectantibody blood level-
dc.subjectantibody detection-
dc.subjectantibody titer-
dc.subjectaqueous humor-
dc.subjectcontrolled study-
dc.subjectcorrelation coefficient-
dc.subjectdiagnostic value-
dc.subjectenzyme linked immunosorbent assay-
dc.subjectimmunofluorescence test-
dc.subjectintermethod comparison-
dc.subjectpolymerase chain reaction-
dc.subjectsensitivity and specificity-
dc.subjectstatistical significance-
dc.subjectAgglutination Tests-
dc.subjectAntibodies, Protozoan-
dc.subjectAqueous Humor-
dc.subjectEnzyme-Linked Immunosorbent Assay-
dc.subjectFluorescent Antibody Technique, Indirect-
dc.subjectImmunoglobulin G-
dc.subjectSwine Diseases-
dc.subjectToxoplasmosis, Animal-
dc.titleEvaluation of IFA, MAT, ELISAs and immunoblotting for the detection of anti-Toxoplasma gondii antibodies in paired serum and aqueous humour samples from experimentally infected pigsen
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionFaculty of Veterinary Medicine-
dc.description.affiliationLaboratório de Protozoologia Departamento de Medicina Veterinária Preventiva Universidade Estadual de Londrina - UEL, P.O. Box 6001, 86050-970 Londrina, PR-
dc.description.affiliationLaboratório de Parasitologia Departamento de Medicina Veterinária e Saúde Animal Universidade de São Paulo - USP, Av. Prof. Orlando Marques Paiva, 87, 05508-000 São Paulo, SP-
dc.description.affiliationImmunoparasitology Laboratory Departamento de Patologia Veterinária Universidade Estadual de São Paulo-UNESP, Via Acesso Prof P Donato Castellane Km 5 s/n, 14884-9000 Jaboticabal, SP-
dc.description.affiliationSection of Veterinary Pathology Department of Basic Veterinary Sciences Faculty of Veterinary Medicine, Helsinki-
dc.description.affiliationUnespImmunoparasitology Laboratory Departamento de Patologia Veterinária Universidade Estadual de São Paulo-UNESP, Via Acesso Prof P Donato Castellane Km 5 s/n, 14884-9000 Jaboticabal, SP-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofResearch in Veterinary Science-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.