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dc.contributor.authorOrtolan, Érika Veruska Paiva-
dc.contributor.authorSpadella, César Tadeu-
dc.contributor.authorCaramori, Carlos Antonio-
dc.contributor.authorMachado, José Lucio Martins-
dc.contributor.authorGregorio, E. A.-
dc.contributor.authorRabello, K.-
dc.identifier.citationExperimental and Clinical Endocrinology and Diabetes, v. 116, n. 4, p. 198-202, 2008.-
dc.description.abstractThe purpose of this study was to investigate if experimental alloxanic diabetes could cause qualitative changes in intestinal anastomoses of the terminal ileum and distal colon in rats, as compared to controls. 192 male Wistar rats, weighing ± 300g were split into four experimental groups of 48 animals each, after 3 months of follow-up: a control group with ileum anastomoses (G1), a control group with colon anastomoses (G2), a diabetic group with ileum anastomoses (G3) and a diabetic group with colon anastomoses (G4). Animals were evaluated and sacrificed on days 4, 14, 21 and 30 after surgery, and fragments of the small and large intestine where the anastomoses were performed were removed. Samples from 6 animals from each sacrifice moment were submitted to ultrastructural analysis of the collagen fibers using a scanning electron microscope and samples from another 6 animals were submitted to histopathology and optical microscopy studies using picrosirius red-staining. Histopathological analysis of picrosirius red-stained anastomosis slides using an optical microscope at 40x magnification showed that the distribution of collagen fibers was disarranged and also revealed a delay in scar tissue retraction. The morphometric study revealed differences in the collagen filled area for the ileum anastomoses 14 days post surgery whereas, in the case of colon anastomoses, differences were observed at days 4 and 30 post surgery, with higher values in the diabetic animals. Ultrastructure analysis of the ileum and colon anastomoses using a scanning electron microscope revealed fewer wide collagen fibers, the presence of narrower fibers and a disarranged distribution of the collagen fibers. We conclude that diabetes caused qualitative changes in scar tissue as well as in the structural arrangement of collagen fibers, what could explain the reduced wound strength in the anastomosis of diabetic animals. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart.en
dc.subjectExperimental diabetes-
dc.subjectIntestinal anastomosis-
dc.subjectcollagen fiber-
dc.subjectalloxan diabetes mellitus-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectanimal tissue-
dc.subjectcontrolled study-
dc.subjectdescending colon-
dc.subjectintestine anastomosis-
dc.subjectpriority journal-
dc.subjectscanning electron microscopy-
dc.subjecttissue structure-
dc.subjectultrastructure analysis and electron microscopy-
dc.subjectWistar rat-
dc.subjectwound healing-
dc.subjectAnastomosis, Surgical-
dc.subjectDiabetes Mellitus, Experimental-
dc.subjectMicroscopy, Electron-
dc.subjectRats, Wistar-
dc.subjectReference Values-
dc.subjectWound Healing-
dc.titleMicroscopic, morphometric and ultrastructural analysis of anastomotic healing in the intestine of normal and diabetic ratsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Surgery Botucatu School of Medicine, Botucatu-
dc.description.affiliationDepartment of Internal Medicine Botucatu School of Medicine, Botucatu-
dc.description.affiliationBioscience Institute Botucatu School of Medicine, Botucatu-
dc.description.affiliationDepartment of Pathology Botucatu School of Medicine, Botucatu-
dc.description.affiliationDepartment of Surgery Botucatu School of Medicine, Rubiao Jr s/n, 18618-730 Botucatu-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofExperimental and Clinical Endocrinology & Diabetes-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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